Development of chemical biology based on allosamidin molecule

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Allosamidin, a chitinase inhibitor, shows anti-asthmatic and chitinase production promoting activities toward mammals and Streptomyces bacteria, respectively. Allosamidin photo-affinity probe was used to identify a chitinase-like protein as a key allosamidin-binding molecule for anti-asthmatic activity of allosamidin. Expression systems to obtain recombinant candidate proteins for allosamidin's target molecule including the chitinase-like protein were constructed. Gene cluster responsible for allosamidin biosynthesis was obtained, which is useful for investigating the role of allosamidin in environment.