Development of renin inhibitor screening system and structure-function relationship of renin inhibitor(s) from food stuffs

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We succeeded effective production of recombinant human renin by baculovirus-insect cell expression system. Using recombinant human renin as a target enzyme, we found renin inhibitory activity in soybean and isolated soybean renin inhibitor. The physico-chemical data on the isolated inhibitor were identical with those of soyasaponin I. In addition, we studied the effects of various saponins on recombinant human renin activity and found that so-called glucuronide saponin which glucuronic acid connected to the third position of sapogenol was renin inhibitory saponins. Furthermore, we found the existence of the renin inhibitor to rice and identified the structure with oleic acid and linoleic acid.