Time and spatial regulation of the embryo implantation at fetomaternal interface ; the resolution of habitual and spontaneous abortion.

本研究旨在阐明小鼠胚胎着床的机制。胚胎着床过程分为2部分；胚胎附着于子宫内膜表面之前的胚胎间距，以及附着后子宫内膜的重塑。除了这些分子网络调控胚胎着床的分析，技术的发展，破坏发现的分子网络，进行了。在胚胎间距的研究中，胚胎附着的预测区域在胚胎从输卵管降至子宫角之前就已经确定了。此外，我们还发现，在子宫内膜重塑过程中，LIF调控孕酮通路和Agrin-acetylcholine通路，而这些通路对于神经-肌肉连接处的功能是必不可少的。最后，本研究发明了一种非常简便的破坏胚胎着床的方法。这项研究将促进胚胎植入研究的快速和广泛发展。

This study aimed to clarify the mechanism of embryo implantation in mice. The process of embryo implantation is divided into 2 portions ; spacing of the embryo before attachment of the embryo to the surface of the endometrium, and remodeling of the endometrium after attachment. In addition to these analysis of molecular networks regulating embryo implantation, development of the technique which disrupt the molecular network discovered, was performed. In the study of embryo spacing, the predicted regions where the embryo attaches, were already determined before the embryo descended from the oviduct to the uterine horn. Also, it was revealed about remodeling of the endometrium that LIF regulates the progesterone pathway, and Agrin-acetylcholine pathway, which is indispensable for the function at the neuro-muscular junction. Finary, very easy method to disrupt embryo implantation was invented in this study. This study must promote rapid and wide development of investigation in embryo implantation.