Time and spatial regulation of the embryo implantation at fetomaternal interface ; the resolution of habitual and spontaneous abortion.

本研究旨在阐明小鼠胚胎着床的机制。胚胎着床的过程分为两个部分：胚胎附着到子宫内膜表面之前的胚胎间距和附着后子宫内膜的重塑。除了这些对调节胚胎植入的分子网络的分析外，还进行了破坏已发现的分子网络的技术的发展。在胚胎间距的研究中，在胚胎从输卵管下降到子宫角之前，就已经确定了胚胎附着的预测区域。此外，在子宫内膜重塑方面，LIF调节孕酮途径，以及对神经-肌肉连接功能不可或缺的Agin-乙酰胆碱途径。本研究发明了一种非常简便的阻断胚胎着床的方法。本研究必将推动胚胎着床研究的快速、广泛发展。

This study aimed to clarify the mechanism of embryo implantation in mice. The process of embryo implantation is divided into 2 portions ; spacing of the embryo before attachment of the embryo to the surface of the endometrium, and remodeling of the endometrium after attachment. In addition to these analysis of molecular networks regulating embryo implantation, development of the technique which disrupt the molecular network discovered, was performed. In the study of embryo spacing, the predicted regions where the embryo attaches, were already determined before the embryo descended from the oviduct to the uterine horn. Also, it was revealed about remodeling of the endometrium that LIF regulates the progesterone pathway, and Agrin-acetylcholine pathway, which is indispensable for the function at the neuro-muscular junction. Finary, very easy method to disrupt embryo implantation was invented in this study. This study must promote rapid and wide development of investigation in embryo implantation.