Does low-gravity environment change the genome and morphology in the living organisms?

低重力环境会改变生物体的基因组和形态吗？

• 批准号: 24650232
• 负责人: HONDO Eiichi
• 金额: $ 2.5万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650232/
• 关键词: 低重力; マウス; エピジェネティクス; 次世代効果; 次世代

This study aimed to clarify the effect of low-gravity on the vertebrates by mimicking its environment on the earth with realizing 0.58g. After the ICR mice were exposed to the low-gravity environment for 2 weeks, the weights of mice differed between the experimental group (low-gravity) and the control. They tended to decrease in the mice of the experimental group compared to the control. The methylation patterns of testicular genomic DNA in both groups were compared using chromatin immunoprecipitation method (ChIP), followed by the next generation sequencing. The contig-sequences by the sequencing corresponded to those inside or nearby 18 genes. Some of these genes are predicted Alzheimer related.

本研究旨在通过模拟地球上0.58g的低重力环境，阐明低重力对脊椎动物的影响。ICR小鼠暴露于低重力环境2周后，实验组（低重力）和对照组小鼠的体重存在差异。与对照组相比，实验组小鼠中的它们倾向于减少。采用染色质免疫沉淀法（ChIP）比较两组睾丸基因组DNA甲基化模式，然后进行下一代测序。测序结果表明，重叠群序列与18个基因内部或附近的重叠群序列相对应。其中一些基因被预测与阿尔茨海默病有关。

项目成果

The complete control of murine pregnancy from embryo implantation to parturition

小鼠妊娠从胚胎着床到分娩的完全控制

• 发表时间: 2012
• 期刊: Reproduction
• 影响因子: 3.8
• 作者: Terakawa J;Watanabe T;Obara R;Sugiyama M;Inoue N;Ohmori Y;Hosaka YZ;Hondo E.
• 通讯作者: Hondo E.

The effect of IL-11 and CNTF on embryo implantation

IL-11和CNTF对胚胎着床的影响

• 发表时间: 2012
• 作者: Terakawa J;Watanabe T;Obara R;Sugiyama M;Inoue N;Ohmori Y;Hosaka YZ;Hondo E.;小林・早川・井上・大森・本道;小林・大森・井上・寺川・本道;Suga/Terakawa/Sugiyama/Ohmori/Inoue/Hon do;Kobayashi/Terakawa/Ohmori/Inoue/Hondo;Nii/Terakawa/Ohmori/Inoue/Hondo;Tajima/Terakawa/Ohmori/Inoue/Hondo
• 通讯作者: Tajima/Terakawa/Ohmori/Inoue/Hondo

The contribution of leukemia inhibitory factor (LIF) for embryo implantation differs among strains of mice

• DOI: 10.1016/j.imbio.2014.03.011
• 发表时间: 2014-07-01
• 期刊: IMMUNOBIOLOGY
• 影响因子: 2.8
• 作者: Kobayashi, Ryosuke;Terakawa, Jumpei;Hondo, Eiichi
• 通讯作者: Hondo, Eiichi

The regulation of embryo spacing by Ihh, BMPs and Wnts in murine uterus

Ihh、BMPs和Wnts对小鼠子宫胚胎间距的调节

• 发表时间: 2012
• 作者: Terakawa J;Watanabe T;Obara R;Sugiyama M;Inoue N;Ohmori Y;Hosaka YZ;Hondo E.;小林・早川・井上・大森・本道;小林・大森・井上・寺川・本道;Suga/Terakawa/Sugiyama/Ohmori/Inoue/Hon do;Kobayashi/Terakawa/Ohmori/Inoue/Hondo
• 通讯作者: Kobayashi/Terakawa/Ohmori/Inoue/Hondo

In vivoエレクトロポレーション法を用いたマウス子宮内膜への遺伝子導入

使用体内电穿孔方法将基因转移到小鼠子宫内膜中

• 发表时间: 2013
• 作者: Terakawa J;Watanabe T;Obara R;Sugiyama M;Inoue N;Ohmori Y;Hosaka YZ;Hondo E.;小林・早川・井上・大森・本道
• 通讯作者: 小林・早川・井上・大森・本道