Identification of canine hair follicle stem cells, reconstruction Of hair follicle and its application to wound healing

犬毛囊干细胞的鉴定、毛囊重建及其在伤口愈合中的应用

• 批准号: 20380171
• 负责人: IWASAKI Toshiroh
• 金额: $ 11.07万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380171/
• 关键词: 犬; 幹細胞; 皮膚; 毛包; 創傷治癒

This research project demonstrated that canine hair follicle stem cells are located in a bulge area of hair follicles and high proliferation potency in the hair follicle stem cells. Stem cell marker proteins, K15, follistatin were stained intensely in the bulge area and related genes such as SOX9, LHX2, TCF4 and NFATC1 were expressed in hair follicle cells of bulge area. In vivo transplant of canine hair follicle stem cells with dermal papilla cells to nude mice successfully reconstituted the hair follicle and its adnexa in nude mouse skin. These results may give a new insight not only to canine hair follicle biology but also to human medicine.

本研究项目证明了犬毛囊干细胞位于毛囊的隆起区域，具有较高的增殖潜能。干细胞标志蛋白K15、卵泡抑素在隆突区呈强阳性表达，相关基因SOX 9、LHX 2、TCF 4、NFATC 1在隆突区毛囊细胞中表达。将犬毛囊干细胞和毛乳头细胞移植到裸鼠体内，成功地在裸鼠皮肤上重建了毛囊及其附件。这些结果可能不仅对犬毛囊生物学而且对人类医学提供新的见解。

项目成果

Canine bulge cells share intrinsic properties with human bulge cells and provide a novel model for human hair follicles stem cell research.

犬凸出细胞与人类凸出细胞具有相同的内在特性，为人类毛囊干细胞研究提供了一种新的模型。

• 发表时间: 2010
• 作者: Kobayashi T;Iwasaki T;Amagai M;Ohyama M
• 通讯作者: Ohyama M

Canine bulge cells share intrinsic proterties with human bulge cells abd provide a novel model for human hair follicle stem cell research

犬凸出细胞与人类凸出细胞具有相同的内在特性，为人类毛囊干细胞研究提供了一种新模型

• 发表时间: 2010
• 作者: Kobayashi T;Iwasaki T;Amagai M;Ohyama M
• 通讯作者: Ohyama M

Molecular biological and immunohistological characterization of canine dermal papilla cells and the evaluation of cutaneous conditions.

犬真皮乳头细胞的分子生物学和免疫组织学表征以及皮肤状况的评估。

• 发表时间: 2011
• 期刊: Veterinary Dermatology in press
• 作者: Kobayashi T;Fujisawa A;Amagai A;Iwasaki T;Ohyama M
• 通讯作者: Ohyama M

Molecular biological and immunohistopathological characterization of canine dermal papilla cells and the evaluation of cutaneous conditions

犬真皮乳头细胞的分子生物学和免疫组织病理学表征以及皮肤状况的评估

• 发表时间: 2011
• 期刊: Veterinary Dermatology
• 影响因子: 1.4
• 作者: Kobayashi T;Iwasaki T;Ohyama T;et al.
• 通讯作者: et al.

Canine Follicle Stem Cell Candidates Reside in the Bulge and Share Characteristic Features with Human Bulge Cells

• DOI: 10.1038/jid.2010.68
• 发表时间: 2010-08-01
• 期刊: JOURNAL OF INVESTIGATIVE DERMATOLOGY
• 影响因子: 6.5
• 作者: Kobayashi, Tetsuro;Iwasaki, Toshiroh;Ohyama, Manabu
• 通讯作者: Ohyama, Manabu