レチノイン酸応答性の新規機能性RNAの同定による肝細胞癌の診療への応用

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It is important to clarify the mechanisms by which retinoid signaling prevents the hepatocarcinogenesis in detail. In the present study, genome-wide analysis of the downstream genes controlled by RA signaling was done by means of in silico and in vitro screenings. First, the in silico approach was used to pick up rnaClusters with RA responsive element (RARE) in their upstream regions, providing 201 rnaClusters (hereafter referred to as Target RNA of Retinoic Acid, TRRA). One hundred eleven of those TRRAs were known as Refseq Genes while the others were unknown naClusters. Next,we screened 201 TRRAs with chromatin immunoprecipitation and quantitative RT-PCR. As a result, it was suggested that the expression of 27 TRRAs was regulated through ATRA-dependent recruitment of retinoic acid eceptors to their RAREs. These twenty-seven TRRAs identified in this study may be useful targets of liver cancer treatment.