Imaging analysis of impaired insulin exocytosis in type2 diabetes mellitus

越来越明显的是，在胰岛素释放受损中观察到的β细胞功能障碍是糖尿病患者进展的必要因素。β细胞中至少有两种胰岛素分泌颗粒，RRP负责初始（第一阶段）胰岛素释放，第二个储备池负责更长的（第二阶段）胰岛素释放。目前还不清楚这两种类型的胰岛素颗粒是如何参与胰岛素释放受损的。在本研究中，我们得到了以下结果。1) β细胞PDK1-Akt信号通路介导第二阶段胰岛素释放的增强。Akt信号似乎参与了肌球蛋白va介导的肌动蛋白网络；2) α - 6失活促进胰岛素释放。3) CDKAL1，控制第一期胰岛素释放。

It is becoming apparent that beta cell dysfunction observed in impaired insulin release is the essential element in the progression of diabetic patients. There are at least two populations of insulin secretory granules in beta cell, the RRP that is responsible for the initial(first phase) insulin release and a second reserve pool that is responsible for a more prolonged(second phase) insulin release. For now, it is still determined how these two types of insulin granules are involved in impaired insulin release In this study, we obtained the following results. 1) PDK1-Akt signaling pathway in beta cell mediated the potentiation of insulin release in the second phase. Akt signals seem to be involved in myosin Va-mediated actin network ; 2) Galpha o inactivation enhances insulin release. 3) CDKAL1, controls 1st phase insulin release.