Autologous Bone Marrow Stromal Cell Transplantation for Stroke: Steps to Standardize the Clinical Protocols

用人血小板裂解液（hPL）代替胎牛血清（FCS）培养人骨髓基质细胞（BMSCs）。用酶联免疫吸附法测定其生长因子的产量。用超顺磁性氧化铁（SPIO）标记骨髓间充质干细胞。建立大鼠永久性局灶性缺血模型，并在损伤后7天将骨髓间充质干细胞注射到同侧纹状体。用hPL和FCS培养的骨髓间充质干细胞在细胞增殖方面没有差异。BMSC-hPL产生的BDNF、HGF、NGF、PDGF-BB和tgf - β均不少于BDNF、HGF、NGF、PDGF-BB和tgf - β。Rotarod试验显示缺血后运动功能恶化，骨髓间充质干细胞移植可促进其恢复。MRI显示spio -骨髓间充质干细胞向病变方向迁移。18F-FDG PET显示葡萄糖利用的恢复得到促进。hPL在骨髓间充质干细胞扩增中可能是有价值和安全的。生物成像技术在骨髓间充质干细胞移植治疗脑卒中方面的应用也很有价值。

Human bone marrow stromal cells (BMSCs) were cultured with human platelet lysate (hPL) instead of fetal calf serum (FCS). Their production of growth factors was quantified with ELISA. The BMSCs were labeled with superparamagnetic iron oxide (SPIO). Rat permanent focal ischemia models were made and BMSCs were injected into the ipsilateral striatum 7 days post-insult. There was no difference in cell proliferation between the BMSCs cultured with hPL and FCS. The BMSC-hPL produced not less amount of BDNF, HGF, NGF, PDGF-BB and TGF-beta1. Rotarod test showed that the motor function deteriorated after ischemia and BMSC transplantation enhanced the recovery. MRI demonstrated that the SPIO-BMSCs migrated towards the lesion. 18F-FDG PET showed that the recovery of glucose utilization was promoted. The hPL may be valuable and safe in expanding BMSCs. The application of bio-imaging techniques are also valuable for BMSC transplantation against stroke.