Autologous Bone Marrow Stromal Cell Transplantation for Stroke: Steps to Standardize the Clinical Protocols

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Human bone marrow stromal cells (BMSCs) were cultured with human platelet lysate (hPL) instead of fetal calf serum (FCS). Their production of growth factors was quantified with ELISA. The BMSCs were labeled with superparamagnetic iron oxide (SPIO). Rat permanent focal ischemia models were made and BMSCs were injected into the ipsilateral striatum 7 days post-insult. There was no difference in cell proliferation between the BMSCs cultured with hPL and FCS. The BMSC-hPL produced not less amount of BDNF, HGF, NGF, PDGF-BB and TGF-beta1. Rotarod test showed that the motor function deteriorated after ischemia and BMSC transplantation enhanced the recovery. MRI demonstrated that the SPIO-BMSCs migrated towards the lesion. 18F-FDG PET showed that the recovery of glucose utilization was promoted. The hPL may be valuable and safe in expanding BMSCs. The application of bio-imaging techniques are also valuable for BMSC transplantation against stroke.