The role of miRNA in the pain mechanisms

在目前的研究中，我们研究了多种mirna的功能，重点关注那些被认为是GABAA α 5受体（Gabra5）调控的潜在靶点。该方法鉴定了六个候选的mirna，它们是Gabra5调控的候选mirna （miR 598-3p、miR541、miR378、miR219-2-3p、miR223和miR346）。荧光素酶测定证实，这6个mirna靶向Gabra5中的特定序列。接下来，我们检查了这些miRNAs是否在蛋白水平上降低了Gabra5的表达。我们发现只有miR378和miR541负调控海马神经元中的Gabra5蛋白。Gabra5在认知和记忆中起着重要作用。通过增加Gabra5的表达水平，我们可能能够改善患有认知问题和记忆功能障碍的阿尔茨海默病患者的症状。miR378和miR541抑制剂是这种推测的新疗法的潜在靶点。

In the current study, we investigated the function of multiple miRNAs, focusing on those that are considered potential targets for GABAA alpha 5 receptor (Gabra5) regulation.The in silico approach identified six miRNAs that were candidates for Gabra5 regulation (miR 598-3p, miR541, miR378, miR219-2-3p, miR223, and miR346). Luciferase assays confirmed that the six miRNAs targeted specific sequences within Gabra5. We next examined whether these miRNAs reduced Gabra5 expression at the protein level. We found that only miR378 and miR541 negatively regulated Gabra5 protein in hippocampal neurons.Gabra5 plays an important role in cognition and memory. By increasing the expression level of Gabra5, we might be able to ameliorate the symptoms of patients with Alzheimer's disease, who suffer from cognitive problems and memory dysfunction. Inhibitors of miR378 and miR541 are potential targets for this putative new therapy.