Functional analysis for molecular interaction through a new member of the immunoglobulin superfamily in tooth germ development

免疫球蛋白超家族新成员在牙胚发育过程中分子相互作用的功能分析

• 批准号: 20390466
• 负责人: SAKAI Hidetaka
• 金额: $ 12.31万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390466/
• 关键词: 口腔病理学; 歯の発生・再生; 歯原性幹細胞; 器官培養; 歯胚; 発生・再生; cDNAサブトラクション; in situ hybridization; 免疫組織化学; アンチセンス法; in situ hybhdization

Protogenin(Prtg) has been identified as a gene that is highly expressed in the mouse mandible at embryonic day 10. 5(E10. 5). Prtg is a new member of the deleted in colorectal carcinoma(DCC) family. This study examined the functional implications of Prtg in the developing tooth germ of the mouse lower first molar. Ptrg is preferentially expressed in the early stage of organogenesis. Prtg was widely expressed in the mesenchymal and oral epithelial cells in the E10. 5 mandible. The expression of Prtg after E12. 0 was markedly reduced in the mandible, and was restricted to the area where the tooth bud was likely to be formed. An antisense inhibition assay for Prtg in cultured E10. 5 mandibles showed a significant growth inhibition in the tooth germ. Bmp-4 expression had significantly been decreased in the mouse E10. 5 mandible at 24 hr after Prtg inhibition. These results indicated that the Prtg might be related to the initial morphogenesis of the tooth germ in the mouse lower first molar. In addition, in situ expression of Ifrg15, L21 and ATPase6 genes suggest that these genes participate in the initiation and the development of the tooth germ as well as those of the other craniofacial organs. Other examination results also demonstrated that Tβ4 is involved in the early stage of tooth germ development by regulation of the odontogenesis-related gene expression.

Protogenin（Prtg）已被鉴定为在胚胎第10天在小鼠下颌骨中高度表达的基因。5（E10. 5）。Prtg基因是大肠癌缺失基因家族的新成员。本研究旨在探讨Prtg在小鼠下第一磨牙牙胚发育过程中的作用。Ptrg在器官发生的早期优先表达。Prtg在E10的间充质细胞和口腔上皮细胞中广泛表达。5下颌骨。Prtg在E12. 0在下颌骨中明显减少，并且仅限于可能形成牙芽的区域。培养的E10中Prtg的反义抑制测定。5个下颌骨在牙胚中表现出明显的生长抑制。BMP-4表达在小鼠E10中显著降低。5下颌骨在Prtg抑制后24小时。这些结果提示Prtg可能与小鼠下第一磨牙牙胚的早期形态发生有关。此外，Ifrg 15、L21和ATPase 6基因的原位表达表明，这些基因参与了牙胚以及其他颅面器官的起始和发育。其他研究结果也表明，Tβ4通过调控牙胚发生相关基因的表达，参与了牙胚发育的早期阶段。

项目成果

Antimicrobial and antifungal effects of tissue conditioners containing a photocatalyst

含有光催化剂的组织调理剂的抗菌和抗真菌作用

• DOI: 10.4012/dmj.2010-203
• 发表时间: 2011
• 期刊: Dental Materials Journal
• 影响因子: 2.5
• 作者: M.Uchimaru;H.Sakai;et al
• 通讯作者: et al

Protogenin, a new member of the immunoglobulin superfamily, is implicated in the development of the mouse lower first molar.

原蛋白是免疫球蛋白超家族的新成员，与小鼠下部磨牙的发展有关。

• DOI: 10.1186/1471-213x-10-115
• 发表时间: 2010-11-25
• 期刊: BMC developmental biology
• 作者: Takahashi KF;Kiyoshima T;Kobayashi I;Xie M;Yamaza H;Fujiwara H;Ookuma Y;Nagata K;Wada H;Sakai T;Terada Y;Sakai H
• 通讯作者: Sakai H

Self-cleaning Ability of a Denture Base Material containing a Photocatalys

含有光催化剂的义齿基托材料的自清洁能力

• 发表时间: 2008
• 期刊: Dent mater J
• 影响因子: 2.5
• 作者: Cheng Y;Sakai T;Moroi R;Nakagawa M;Sakai H;Ogata T and Terada Y
• 通讯作者: Ogata T and Terada Y

A possible suppressive role of galectin-3 in upregulated osteoclastogenesis accompanying adjuvant-induced arthritis in rats

• DOI: 10.1038/labinvest.2008.111
• 发表时间: 2009-01-01
• 期刊: LABORATORY INVESTIGATION
• 影响因子: 5
• 作者: Li, Yin-Ji;Kukita, Akiko;Kukita, Toshio
• 通讯作者: Kukita, Toshio

歯胚発生過程における膜蛋白Protogeninの発現と歯胚発育への関与

牙胚发育过程中膜蛋白Protogenin的表达及其参与牙胚发育的过程

• 发表时间: 2008
• 作者: 清島保;小林家吉;坂井英隆
• 通讯作者: 坂井英隆