Morphological and molecular biological investigations on the relationship between tumor-related gene alteration, and the generation and growth of oral cancer.

肿瘤相关基因改变与口腔癌发生、生长关系的形态学和分子生物学研究。

• 批准号: 09470392
• 负责人: SAKAI Hidetaka
• 金额: $ 8.7万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09470392/
• 关键词: Oral Cancer; Epstein-Barr virus; human papilloma virus; p53 onco-suppressor gene; EGF-receptor; basement membrane protein; cytoskeletal molecule; doc-1; 唾液腺腫瘍; p53; 細胞骨格; 上皮増殖因子; 唾液腺癌; P53癌抑制遺伝子; EBウイルス; ヒトパピローマウイルス; 浸潤、転移; 分子生物学; 免疫組織化学

Oral squamous cell carcinoma (OSCC) were evaluated for the prevalence of Epstein-Barr virus (EBV) infection. EBV DNA was detected in 7 out of 46 samples. A follow-up study showed no recurrence or death to occur in the EBV-positive patients, which thus suggested a good prognosis of EBV-positive OSCC patients. The prevalence of human papillomaviruses (HPV) 16 and 18 infection, and p53 mutation in OSCC were also examined. A higher incidence of HPV16 and 18 infections than previous studies was showed in this study. The mutanogenic effect of HPV16 on the p53 mutation.The relationship between immunohistochemical localization of Cathepsin D (CD). proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGF-R) in 65 cases of breast carcinoma was examined, and suggest that both the CD parameters and EGF-R are valuable indicators for predicting the biological behavior of human breast carcinoma. The alteration of EGF-R was also examined in the OSCC, and showed that a novel … More truncated form of EGF-R gene exists in the OSCC.The immunohistochemical localization of laminin, collagen type IV and heparan sulfate proteoglycan (BMs) were investigated in 78 samples of human colorectal carcinoma. The results indicate that the lower degree of immunostaining ratio of BMs at the parenchymal-stromal border of the invasive margin is related to both the lymph node metastasis and the patient prognosis.,The expression of cytoskeletal molecules in the OSCC cell lines were examined, and showed that the cytoskeletal system in the OSCC cells is related to the malignant phenotype of OSCC cells.p12DOC-1, which is a growth suppressor identified and isolated from normal keratinocytes, was appeared to be related to negative regulation of the cyclin-dependent kinase-mediated phosphorylation of pol-a : primaseSeventeen adenoid cystic carcinomas (ACC) and 27 mucoepidermoid carcinomas (MEC) in the salivary glands were analysed for p53 tumor suppressor gene alteration and protein expression. The occurrence of the p53 gene alteration is less frequent in ACC and MEC than that in other kinds of tumors. All ACC samples exhibiting p53 gene alterations showed recurrence/metastasis, suggesting a poor outcome of these patients. Less

评估口腔鳞状细胞癌（OSCC）中eb病毒（EBV）感染的患病率。46份样本中有7份检测到EBV DNA。随访研究显示ebv阳性患者无复发或死亡，提示ebv阳性OSCC患者预后良好。我们还检测了OSCC中人乳头瘤病毒（HPV） 16和18感染的患病率以及p53突变。本研究显示HPV16和hpv18感染的发生率高于以往的研究。HPV16对p53突变的致突变作用。组织蛋白酶D （CD）免疫组化定位的关系。通过对65例乳腺癌组织中增殖细胞核抗原（PCNA）和表皮生长因子受体（EGF-R）的检测，提示CD参数和EGF-R是预测乳腺癌生物学行为的重要指标。在OSCC中也检测了EGF-R的变化，并表明在OSCC中存在一种新的、更短的EGF-R基因。研究了78例大肠癌组织中层粘连蛋白、IV型胶原和硫酸肝素蛋白多糖（BMs）的免疫组化定位。结果表明，浸润缘实质-间质交界区脑转移瘤免疫染色率较低与淋巴结转移及患者预后有关。对细胞骨架分子在OSCC细胞系中的表达进行了检测，发现OSCC细胞的细胞骨架系统与OSCC细胞的恶性表型有关。p12DOC-1是一种从正常角质形成细胞中分离出来的生长抑制因子，似乎与细胞周期蛋白依赖性激酶介导的pol-a磷酸化的负调控有关。我们分析了唾液腺中17例腺样囊性癌（ACC）和27例粘液表皮样癌（MEC）的p53肿瘤抑制基因改变和蛋白表达。与其他肿瘤相比，ACC和MEC中p53基因改变的发生率较低。所有显示p53基因改变的ACC样本均出现复发/转移，提示这些患者预后较差。少

项目成果

Ieyoshi Kobayashi et al.: "Salivary Duct Carcinoma with Sebaceous Cell Differentiation arising from Parotid Gland : Histological, Immunohistochemical and Ultrastructural Analyses of a Case"Oral Medicine and Pathology. 2・2. 89-93 (1997)

Ieyoshi Kobayashi 等：“腮腺引起的唾液管癌：组织学、免疫组织化学和超微结构分析”口腔医学和病理学 2・2。

Kou Matuo et al.: "p12^<DOC-1>, a growth suppressor, associates with DNA polymerase α/primase."The FASEB Journal. 14. 1318-1324 (2000)

Kou Matuo 等人：“p12^<DOC-1>，一种生长抑制剂，与 DNA 聚合酶 α/引物酶相关。”FASEB 杂志 14. 1318-1324 (2000)

Satoru Shintani et al.: "Intragenic mutation analysis of the human epidermal growth factor receptor (EGFR) gene in malignant human oral keratinocytes."Cancer Research. 59. 4142-4147 (1999)

Satoru Shintani 等人：“恶性人口腔角质形成细胞中人表皮生长因子受体 (EGFR) 基因的基因内突变分析。”癌症研究。

Satoru Shintani: "Intragenic mutation analysis of the human epidermal grwoth factor receptor (EGFR) gene in malignant human oral keratinocytes."Cancer Research. 59. 4142-4147 (1999)

Satoru Shintani：“恶性人类口腔角质形成细胞中人类表皮生长因子受体（EGFR）基因的基因内突变分析。”癌症研究。

S.Shintani et al.: "p12^<DOC-1> is a novel-cyclin-dependent kinase-2 associated protein."Mol.Cell Biol. 20. 6300-6307 (2000)

S.Shintani 等人：“p12^<DOC-1> 是一种新型的细胞周期蛋白依赖性激酶 2 相关蛋白。”Mol.Cell Biol。