Pathological and molecular biological Investigations on the mechanism of generation, proliferation and metastasis of Oral Squamou Cell Carcinoma.

口腔鳞状细胞癌发生、增殖和转移机制的病理和分子生物学研究。

• 批准号: 07457430
• 负责人: SAKAI Hidetaka
• 金额: $ 3.71万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457430/
• 关键词: p53; Epstein-Barr virus; human papilloma virus; PCR; cathepsin D; basement membrane; invasin and metastasis; immunohistochemistry; EBウイルス; 口腔癌; 浸潤・転移; 癌遺伝子; 癌抑制遺伝子; 蛋白分解酵素; 細胞間基質; ウイルス

Relationship between Epstein-Barr (EBV) or human papilloma (HPV) viruses infection and the expression of p53 onco-supressor gene in oral squmaous cell carcinoma was investigated. Mutation of p53 gene was detected in 20 out of 47 cases. Southern blot analysis revealed that 7 cases expressed the Bam H1 region of EBV gene. All Bam H1-positive cases showed LAP-1 protein-positive by means of the immunohistochemical staining, and these positive cells were localized at the periphery of the carcinoma tissue suggesting the relationship between EBV infection and proliferation of carcinoma tissue. HPV gene was detected in 31 cases, and was statistically correlated to the p53 mutation. Reginal lymph node metastasis was statistically higher in cases with HPV in fection.Immunohistochemical localization of cathepsins D and E in human gastric carcinoma showed that these proteases were strongly expressed in carcinoma cells at the advancing mergin, suggesting a close correlation of these proteases with the local invasion of carcinoma cells. Immunohistochemical localization of cathepsins D,EGF-receptor and PCNA in human breast carcinoma also showed that these proteins closely related to the lymph node metatstasis and prognosis of the patients.Basement membrane (BM) proteins such as laminin, Collage type IV and heparan sulfate proteoglycan in the human colorectal carcinoma was investigated by means of immunohistochemistry. Localization of BMs in the carcinoma tissue was statistically related to the lymph node metastasis. Histological invasion pattern at the parenchymal-stromal border was also correlated with the localization of BMs. These results suggest that the invasive pattern was important histological leatures to predict the biological behavier of colorectal carcinomas.

探讨EBV （Epstein-Barr， EBV）和人乳头瘤病毒（human papilloma， HPV）感染与p53抑癌基因表达的关系。47例中有20例检测到p53基因突变。Southern blot分析显示，7例患者表达EBV基因Bam H1区。所有Bam h1阳性病例的免疫组化染色均显示LAP-1蛋白阳性，这些阳性细胞位于癌组织的周围，提示EBV感染与癌组织的增殖有关。31例检出HPV基因，与p53突变有统计学相关性。在感染HPV的病例中，区域淋巴结转移具有统计学意义。对人胃癌组织蛋白酶D和E的免疫组化定位显示，这两种蛋白酶在癌细胞的进退处强烈表达，提示这些蛋白酶与癌细胞的局部侵袭密切相关。人乳腺癌组织蛋白酶D、egf受体和PCNA的免疫组化定位也显示这些蛋白与患者淋巴结转移和预后密切相关。采用免疫组织化学方法研究了人结直肠癌基底膜（BM）蛋白层粘连蛋白（laminin）、IV型胶原蛋白（Collage IV）和硫酸肝素蛋白多糖（heparan sulfate proteogcan）的表达。脑转移瘤在癌组织中的定位与淋巴结转移有统计学意义。实质-间质边界的组织学浸润模式也与脑转移灶的定位相关。这些结果表明，浸润模式是预测结直肠癌生物学行为的重要组织学特征。

项目成果

Yuuji Asaoka: "Relationship between proliferative activity,and tissue differentiation and invasive mode in human oral squamous cell and colorectal carcinomas analyzed by PCNA immunostaining" Fukuoka Acta Medica. 86. 317-324 (1995)

Yuuji Asaoka：“通过 PCNA 免疫染色分析人口腔鳞状细胞和结直肠癌的增殖活性、组织分化和侵袭模式之间的关系”福冈医学学报。

Ieyoshi Kobayashi: "The Proliferative Activity in Oral Epithelial Dysplasia Analyzed by Proliferating Cell Nuclear Antigen Immunostaining asnd Argyrophilic Nucleolar Organizer Region Staining." Human Pathology. 26. 907-913 (1995)

Ieyoshi Kobayashi：“通过增殖细胞核抗原免疫染色和嗜银核仁组织区染色分析口腔上皮发育不良的增殖活性。”

Ieyushi Kobayashi et al.: "The Proliferative Activity in Oral Epithelial Dysplasia Analyzed by Proliferating Cell Nuclear Antigen Immunostaining and Argyophilic Nucleolar Organizer Region Staining." Human Pathol.26(8). 907-913 (1995)

Ieyushi Kobayashi 等人：“通过增殖细胞核抗原免疫染色和嗜银核仁组织区染色分析口腔上皮发育不良的增殖活性。”

Yuuji Asaoka: "Relationship between proliferative activity,and tissue differentiation and invasive mode in human oral squamous cell and colorectal carcinomas analyzed by PCNA immunostaining" Fukuoka Acta Medica. 86・7. 317-324 (1995)

Yuuji Asaoka：“通过PCNA免疫染色分析人口腔鳞状细胞和结直肠癌的增殖活性与组织分化和侵袭模式的关系”福冈医学学报86・7（1995）。

Ieyoshi Kobayashi, et al.: "The Proliferative Activity in Oral Epithelial Dysplasia Analyzed by Proliferating Cell Nuclear Antigen Immunostaining and Argyrophilic Nucleolar Organizer Region Staining" Human Pathology. 26. 907-913 (1995)

Ieyoshi Kobayashi 等人：“通过增殖细胞核抗原免疫染色和嗜银核仁组织区域染色分析口腔上皮发育不良的增殖活性”人类病理学。