Pathological and molecular biological Investigations on the mechanism of generation, proliferation and metastasis of Oral Squamou Cell Carcinoma.

探讨口腔鳞癌中EB病毒（EBV）和人乳头瘤病毒（HPV）感染与p53抑癌基因表达的关系。47例中20例检测到p53基因突变。Southern杂交结果显示7例有EBV基因的BamH 1区表达。免疫组化结果显示，所有Bam H1阳性病例均呈EBV-1蛋白阳性，且阳性细胞位于癌组织的周边，提示EBV感染与癌组织的增殖有关。HPV基因阳性31例，与p53突变相关。组织蛋白酶D和E在人胃癌组织中的免疫组化定位显示，这两种蛋白酶在癌细胞的融合区有强表达，提示它们与癌细胞的局部浸润密切相关。组织蛋白酶D、表皮生长因子受体和增殖细胞核抗原在乳腺癌中的免疫组化定位也表明这些蛋白与乳腺癌的淋巴结转移和预后密切相关。BM在癌组织中的定位与淋巴结转移有关。组织学浸润模式在实质间质边界也与本地化的BM。结果提示，侵袭性是预测大肠癌生物学行为的重要组织学特征。

Relationship between Epstein-Barr (EBV) or human papilloma (HPV) viruses infection and the expression of p53 onco-supressor gene in oral squmaous cell carcinoma was investigated. Mutation of p53 gene was detected in 20 out of 47 cases. Southern blot analysis revealed that 7 cases expressed the Bam H1 region of EBV gene. All Bam H1-positive cases showed LAP-1 protein-positive by means of the immunohistochemical staining, and these positive cells were localized at the periphery of the carcinoma tissue suggesting the relationship between EBV infection and proliferation of carcinoma tissue. HPV gene was detected in 31 cases, and was statistically correlated to the p53 mutation. Reginal lymph node metastasis was statistically higher in cases with HPV in fection.Immunohistochemical localization of cathepsins D and E in human gastric carcinoma showed that these proteases were strongly expressed in carcinoma cells at the advancing mergin, suggesting a close correlation of these proteases with the local invasion of carcinoma cells. Immunohistochemical localization of cathepsins D,EGF-receptor and PCNA in human breast carcinoma also showed that these proteins closely related to the lymph node metatstasis and prognosis of the patients.Basement membrane (BM) proteins such as laminin, Collage type IV and heparan sulfate proteoglycan in the human colorectal carcinoma was investigated by means of immunohistochemistry. Localization of BMs in the carcinoma tissue was statistically related to the lymph node metastasis. Histological invasion pattern at the parenchymal-stromal border was also correlated with the localization of BMs. These results suggest that the invasive pattern was important histological leatures to predict the biological behavier of colorectal carcinomas.