Histopathological Investigation on the mechanism of generation, proliferation and metastasis of Oral Squamou Cell Carcinoma.

新的口腔鳞癌细胞系MISK 81-5的建立及其生物学特性已发表在《日本癌症研究杂志》上。MISK 81-5细胞显示非整倍体染色体，并向培养上清液中释放组织蛋白酶D，该酶可能与癌细胞的侵袭能力有关。经干细胞实验和ELISA检测，该细胞株还产生高水平的粒细胞集落刺激因子。通过将人组织蛋白酶D基因导入该细胞系，对其侵袭能力的改变进行了实验研究。对与细胞表面转化和黏附分子相关的未知抗原抗体的制备也进行了研究。用增殖细胞核抗原免疫组织化学染色和AgNORs染色观察了宫颈异型增生、宫颈原位癌和口腔粘膜的增殖活性。这些研究揭示了宫颈和口腔黏膜上皮异型增生的严重程度与其增殖活性密切相关，阐明了口腔粘膜重度上皮异型增生的主要组织学成分。这些研究的结果发表在《人类病理学》杂志上。对抑癌基因P53在口腔癌前病变中的定位进行了免疫组织化学检测，并用聚合酶链式反应和原位杂交的方法检测了其mRNA和点突变。结果表明，这些蛋白在进展期的癌细胞中有较强的表达，提示它们与癌细胞的局部侵袭和随后的淋巴结转移密切相关。这项研究已经提交给了一家期刊。

Establishment and biological characteristics of a new oral squmaous carcinoma cell line, MISK 81-5, had been published in Japanese Journal of Cancer Research. MISK 81-5 cells showed an aneuploid chromosomal, and released cathepsin D into culture media, which protease is supposed to correlate with invasive potencies of carcinoma cells. This cell line alos produced a high level of granulocyte colony-stimulating factor demonstrated by stem cell assay and ELISA.An experimental study on the alteration of the invasive potency has been performed by transfecting a human cathepsin D cDNA into the cell line. Preparations of antibodies against unknown antigens correlated with transformation and adhesion molecules on the cell surface has been also investigating.Proliferative activities of the dysplasia and carcinoma in situ in the uterine cervix, and oral mucosa were investigated by means of PCNA immunostaining and AgNORs staining. These studies disclosed the close correlation between the proliferative activity and the severity of the dysplasia in uterine cervix and oral mucosa, main histological components related to severe epithelial dysplasia of the oral micosa were clarified. The results of these investigations have been published in Human Pathology. Immunohistochemical examinations on the localization of p53, as a tumor suppressor gene in oral precancerous lesion, as well as detections of its mRNA and point mutations by using PCR and in situ hybridization methods, are proceeding.Immunohistochemical localizations of cathepsins D and E in primary gastric cancer were investigated. The results that these proteases were strongly expressed in carcinoma cells at the advancing mergin suggesting a closely correlation with the local invasion of carcinoma cells and following metastsis to the lymph nodes. This sytudy has been submitted to a journal.