Establishment of a network of model-monkey supply forhuman medical researches

建立人类医学研究模型猴供应网络

• 批准号: 20500371
• 负责人: TSUCHIYA Hideaki
• 金额: $ 2.91万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20500371/
• 关键词: カニクイザル; 人工繁殖; MHC遺伝子; モデル動物; 供給ネットワーク; 室内人工繁殖; 医療研究用モデルザル; 動物供給ネットワーク; データベース; 生殖細胞

Cynomolgus monkeys (Macaca fascicularis) live in a broad range of South-East Asia, and nonhuman primates (NHPs) have emerged as important animal models in infectious diseases and transplantation studies. For those studies, it’s necessitating a more extensive characterization of their major histocompatibility complex (MHC) polymorphic regions and MHC homozygous (homo) cynomolgus monkey would have big advantage for immunological research. We have collected a blood samples from many cynomolgus monkey breeding facilities in Japan and South-East Asia, and MHC gene analysis has been initiated. These animals are bred artificially and it is necessary to establish the system to supply. The supply system of these animals was divided into three stages, and it was established. First stage: It cooperates between with each facility and information-material was shared. Second stage: Establishment the indoor artificial breading system with the materials offered from each facility. Third stage: It supplies in order to use the produced MHC identified monkey for research. In this study, we have demonstrated our model monkey supply system followed by indoor artificial breading system could show viable MHC identified monkey and it could work even at infectious diseases and transplantation studies.

食蟹猴（Macaca fascicularis）广泛分布于东南亚，非人灵长类动物（NHP）已成为传染病和移植研究的重要动物模型。对于这些研究，有必要更广泛地表征其主要组织相容性复合体（MHC）多态性区域，并且MHC纯合（homo）食蟹猴将在免疫学研究中具有很大的优势。我们从日本和东南亚的许多食蟹猴养殖设施收集了血样，并已开始进行MHC基因分析。这些动物都是人工饲养的，有必要建立供应体系。这些动物的供应系统分为三个阶段，并建立起来。第一阶段：它与各设施之间进行合作，并共享信息资料。第二阶段：利用各设施提供的材料，建立室内人工繁殖体系。第三阶段：为利用生产的MHC鉴定猴供研究。在这项研究中，我们已经证明了我们的模型猴供应系统，然后是室内人工繁殖系统可以显示可行的MHC鉴定的猴子，它可以工作，甚至在传染病和移植研究。

项目成果

ガス吸引補助具

气体抽吸辅助装置

• 发表时间: 2009

サル胚性幹細胞、「卵子学」

猴胚胎干细胞，“卵子学”

• 发表时间: 2011
• 作者: Takagishi Y;Okabe E;Chishima Y;Sun XY;Senoo S;Inaba M;Mizumura K;Komatsu Y;Oda SI;Murata Y.;片平清昭;鳥居隆三
• 通讯作者: 鳥居隆三

Torii R Triplet pregnancy in a cynomolgus monkey (Macaca fascicularis) after double embryo transfer

Torii R 双胚胎移植后食蟹猴（Macaca fasciculis）三胞胎妊娠

• 发表时间: 2012
• 期刊: Comparative Medicine
• 影响因子: 0.8
• 作者: Morichika J;Iwatani C;Tsuchiya H;Nakamura S;Sankai T
• 通讯作者: Sankai T

カニクイザルにおける単一割球からのES細胞の樹立

食蟹猴单卵裂球 ES 细胞的建立

• 发表时间: 2008
• 作者: 岡原(成田)純子;山;樹里;岩谷千鶴;土屋英明;鳥居隆三
• 通讯作者: 鳥居隆三

A cynomolgus monkey embryonic stem cell line derived from a single blastomere

来自单个卵裂球的食蟹猴胚胎干细胞系

• 发表时间: 2008
• 期刊: Reproduction, Fertility and Development 20
• 作者: Okahara-Narita J;Yamasaki J;Iwatani C;Tsuchiya H. Torii R
• 通讯作者: Tsuchiya H. Torii R