本研究采用显微和超微结构分析、免疫组织化学、实时荧光定量PCR、转录组测序等技术，研究MPT和FPT条件下扬子鳄胚胎性腺发育不同时期雌激素受体(ERα和ERβ)、雄激素受体 (AR)、孕激素受体(PR)、黄体生成激素(LHR)、卵泡刺激素(FSHR)和促性腺激素释放激素受体( GnRHR)等的定位、基因定量表达及基因调控网络分析。研究内容:(1)确定胚胎发育过程中，雌性和雄性性腺出现分化的具体时间及性腺的结构变化;(2)研究不同的性激素受体在性腺发育及性别分化过程中出现的时间、分布与定位;(3)探讨不同性激素受体基因在性腺发育及性别分化过程中基因表达调控;(4)分析、鉴定与扬子鳄性别决定相关的新候选基因，分析扬子鳄性别分化相关基因调控网络。本研究结果将加深人们对扬子鳄早期精巢或卵巢发育进程的了解，深入理解TSD物种性别分化潜在的分子机制，并为人工繁育调节扬子鳄饲养种群性比提供科学依据。

In this project, we plan to investigate the embryonic gonads of the Chinese alligator hatched under MPT and FPT by using ultrastructure, immunohistochemistry, quantitative real-time PCR (qRT-PCR) and RNA-Seq techniques. The gonad samples were collected during various period of embryonic development, and then we analyze the distribution, quantitative expression of ERα and ERβ, AR，PR，LHR，FSHR and GnRHR receptors for each temperature conditions, and also analyze the Gene regulatory network of sex determination. This project will present more knowledge of early embryonic development and sex differentiation. The researches are try to (1) to determine the time of Female and male gonads and the change of gonads of the Chinese alligator; (2) to research the distribution and location of sex hormone receptors; (3) to study the gene expression of various sex hormone receptors during the period of differentiation gonads and sex differentiation; (4) to analyze and identify the new candidate genes and gene regulatory networks related with sex determination of the Chinese alligator.　The results will promote to make understanding of early gonads developmental process of the Chinese alligator and the potential molecular mechanism of TSD species, and meanwhile, provide the scientific basis for controlling the sex ratio of captive population by artifitial breeding.