The tumorigenicity and transmissible genetic risk in mice under the Chernobyl simulation

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To simulate the radiocontamination in middle contaminated areas of Belarus (1997, 2005), mice were maintained for 8 months in the radioisotope facility with free access to drinking water containing ^<137>CsCl (0Bq/ml, 10Bq/ml and 100Bq/ml).Mice were assessed the long term low dose rate and low dose internal and external radiation effect by the quantitative measurement of the contaminated radionuclides-induced DNA double-strand breaks by γ-H2AX foci in the organs. The micro nuclei test and the tumorigenicity of mouse lung were also examined under the same condition.The number of γ-H2AX focus per hepatic cell was 1.4 (0Bq/ml), 9.5(10Bq/ml), 10.7(100Bq/ml), respectively. It was suggested that the genome damage caused internal low dose ^<137>Cs radiation were occurring chronically even in low dose contaminated area. But no significant difference was observed in the micro nuclei test. In addition, progress data (by 6 animals in each dose groups) is showing that the number of lung tumors per mouse induced by urethane under the same condition is 36.8(368/10) (0 Bq/ml), 44.1 (441/10) (10Bq/ml), 44.1 (441/10) (100Bq/ml), and the average of tumor diameter (mm) is 1.64, 1.67, 1.68, respectively.