An attempt to develop a biodosimeter for detecting low dose and low dose rate radiation

开发检测低剂量和低剂量率辐射的生物剂量计的尝试

• 批准号: 09680521
• 负责人: NAKAJIMA Hiroo
• 金额: $ 2.11万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680521/
• 关键词: SCID; Cell culture; Mouse; Biodosimater; Double strand break; DNA repair; Low dose radiation; Potentially lethal damage

We attempted to develop a new biodosimeter to detect the low dose and low dose rate radiation.Normal (wild type cells) and double-strand break repair deficient murine cells (SCID cells) were exposed to gamma-rays at low and high dose rates with dose fractionation and with different recovery times, in order to examine the dose rate effectiveness and repair of sublethal and potentially lethal damage.In addition, 5-fluorouracil (5-FU), a commonly used drug for cancer therapy, was a]so used, because it produces double-strand breaks.SOlD cells showed deficiency in repairing DNA damage induced by 5-FU or gamma-rays even after dose fractionation and after 24h recovery periods, while the wild type cells showed an apparent repair ability on DNA damage after 5-FU treatment or gamma-rays exposure.We conclude that the SCID cell is useful system as a biodosimeter for detecting the damage of low dose radiation.Now, we are trying to establish a method to count the number of double-strand break points in a SCID cell.

我们尝试研制一种新的生物剂量计，用于检测低剂量和低剂量率的辐射。（野生型细胞）和双链断裂修复缺陷型鼠细胞（SCID细胞）以低和高剂量率暴露于具有剂量分级和不同恢复时间的γ射线，以检查剂量率有效性和亚致死性和潜在致死性损伤的修复。5-氟尿嘧啶（5-FU）是一种常用的癌症治疗药物，由于其产生双链断裂而被如此使用。SOlD细胞在修复由5-FU或γ射线诱导的DNA损伤方面表现出缺陷，即使在剂量分割后和24小时恢复期后，而野生型细胞在5-FU处理或γ-FU处理后显示出明显的DNA损伤修复能力，结果表明，SCID细胞是一种检测低剂量辐射损伤的生物剂量计系统，我们正试图建立一种方法来计数SCID细胞中双链断裂点的数目。

项目成果

Nakajima,H.,et al.: "Radionuclides carved on the annual rings^n of a tree near Chernobyl" Health Physics. 74. 265-267 (1998)

Nakajima,H.,et al.：“切尔诺贝利附近一棵树的年轮^n上刻有放射性核素”健康物理学。

Fukuda, K., et al.: "Morphology and function of human benign tumors and normal thyroid tissues maintained in severe combined immunodeficient mice." Cancer Letters. 132. 153-158 (1998)

Fukuda, K. 等人：“在严重联合免疫缺陷小鼠中维持的人类良性肿瘤和正常甲状腺组织的形态和功能。”

Li,L., et.al.: "Dose-rate effectiveness and potentially lethal damage repair in normal and double-strand-break repair deficient murine cells by γ rays and 5-fluorouracil." Cancer Letters. 123. 227-323 (1998)

Li, L., et.al.：“γ 射线和 5-氟尿嘧啶对正常和双链断裂修复缺陷小鼠细胞的剂量率有效性和潜在致命损伤修复。”123. 227-323（癌症快报） 1998）

Nakajima,H., et al.: "Radionuclides carved on the annual rings of a tree near Chernobyl." Health Physics. 74. 265-267 (1998)

Nakajima,H. 等人：“切尔诺贝利附近一棵树的年轮上刻有放射性核素。”

Nomura,T.,et al.: "Induction of cancer.actinic keratosis,and specific p53 mutation by UVB light in human skin maintained in severe commbined immunodeficient mice." Cancer Res.57. 2081-2084 (1997)

Nomura,T.,et al.：“在严重联合免疫缺陷小鼠中维持人类皮肤中 UVB 光诱导癌症、光化性角化病和特异性 p53 突变。”