Specific degradation of peptides with hydrogen radical and its application to protein chemistry

在水溶液中加入苯乙胺作为释氢剂，超声超声水解多肽。肽的超声水解在特性上不同于常规酸水解，即，脯氨酸残基的裂解和作为副反应的脱水。结合巯基加成的超声分解使得确定肽的氨基酸序列成为可能。此外，在真空条件下的紫外激光光子辐照实验中，找到了新的释放氢原子的试剂。

The ultrasound sonolytic hydrolysis of peptides was performed with added benzenethiol as a hydrogen-releasing reagent in aqueous solution. The ultrasound hydrolysis of peptides differed from that due to conventional acid hydrolysis in characteristics, i.e., cleavage at proline residue and dehydration as a side reaction. The sonolysis combined with the thiol addition made it possible to determine the amino-acid sequence of peptides. Furthermore, new reagent for releasing hydrogen atoms was found out in the experiment of the irradiation of ultraviolet laser photon in vacuum condition.