Control of Interface in Cancer Cell Membranes and Inhibitory Effects of Three-component Hybrid Liposomes Including Sugar Surfactant

杂化脂质体（HL）可以通过在缓冲溶液中超声处理囊泡和胶束分子来制备。HL的物理性质，如大小，形状，膜流动性，和相分离的温度可以通过改变组成和组成比例来控制。我们采用L-α-二肉豆蔻酰磷脂酰胆碱（DMPC）、聚氧乙烯十二烷单月桂酸酯（Tween 20）和癸基-β-乳吡喃糖苷（LactC_<10>）（DMPC：Tween 20：LactC_<10>= 65：7：28）组成的三组分杂化脂质体（THL）进行了肿瘤研究，结果如下：（A）<10>从β-乳糖八<10>乙酸酯（Ac-Lact）经糖基化和脱乙酰化得到的癸基-β-乳七乙酸酯（Ac-LactC_）成功地合成了LactC_。(B)含乳糖表面活性剂的三组分混合脂质体（HL/LactC_<10>）对人肝癌细胞（Hep-G2、Huh-7）的IC_（50）值小于二组分混合脂质体（HL）。(C)HL/LactC_1固定水层厚度<10>约为HL的2倍。结果提示HL/LactC_2对肿瘤细胞的抑制作用<10>可能与肿瘤细胞的水化有关。(D)HL/LactC_2<10>对Hep-G_2和Huh-7细胞均有诱导凋亡作用，从而证实了具有特异性固定水层的THL抑制肝癌细胞生长与水化之间关系的重要性。

Hybrid liposomes (HL) can be prepared by sonication of vesicular and micellar molecules in a buffer solution. The physical properties of HL such as size, shape, membrane fluidity, and the temperature of phase separation can be controlled by changing the constituents and compositional ratio. We have employed three-component hybrid liposomes (THL) composed of L-α-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene sorbitane monolaurate (Tween 20), and decyl-β-lactopranoside (LactC_<10>) (DMPC : Tween 20 : LactC_<10> = 65 : 7 : 28) for the research of cancers and interesting results are as follows;(A) LactC_<10> was successfully produced from decyl-β-lactoheptaacetate (Ac-LactC_<10>), which was obtained by glycosylation and deacetylation of β-lactoseoctaacetate (Ac-Lact).(B) IC50 values of three-component hybrid liposomes including lactose surfactants (HL/LactC_<10>) for human hepatoma (Hep-G2, Huh-7) cells were smaller than those of two-component hybrid liposomes (HL).(C) The thickness of fixed aqueous layer (TFAL) of HL/LactC_<10> was about twice that of HL. The result suggests that the inhibitory effects of HL/LactC_<10> could be related to hydration in tumor cells.(D) Induction of apoptosis by HL/LactC_<10> for Hep-G2 and Huh-7 cells were obtained.Thus, this study demonstrated the importance of relation between hydration and inhibition of the growth of hepatoma cells using THL having specific fixed aqueous layers.