Control of Interface in Cancer Cell Membranes and Inhibitory Effects of Three-component Hybrid Liposomes Including Sugar Surfactant

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Hybrid liposomes (HL) can be prepared by sonication of vesicular and micellar molecules in a buffer solution. The physical properties of HL such as size, shape, membrane fluidity, and the temperature of phase separation can be controlled by changing the constituents and compositional ratio. We have employed three-component hybrid liposomes (THL) composed of L-α-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene sorbitane monolaurate (Tween 20), and decyl-β-lactopranoside (LactC_<10>) (DMPC : Tween 20 : LactC_<10> = 65 : 7 : 28) for the research of cancers and interesting results are as follows;(A) LactC_<10> was successfully produced from decyl-β-lactoheptaacetate (Ac-LactC_<10>), which was obtained by glycosylation and deacetylation of β-lactoseoctaacetate (Ac-Lact).(B) IC50 values of three-component hybrid liposomes including lactose surfactants (HL/LactC_<10>) for human hepatoma (Hep-G2, Huh-7) cells were smaller than those of two-component hybrid liposomes (HL).(C) The thickness of fixed aqueous layer (TFAL) of HL/LactC_<10> was about twice that of HL. The result suggests that the inhibitory effects of HL/LactC_<10> could be related to hydration in tumor cells.(D) Induction of apoptosis by HL/LactC_<10> for Hep-G2 and Huh-7 cells were obtained.Thus, this study demonstrated the importance of relation between hydration and inhibition of the growth of hepatoma cells using THL having specific fixed aqueous layers.