Control of Interface in Cancer Cell Membranes and Inhibitory Effects of Three-component Hybrid Liposomes Including Sugar Surfactant

含糖表面活性剂的三组分杂化脂质体对癌细胞膜界面的控制和抑制作用

• 批准号: 20560732
• 负责人: MATSUMOTO Yoko
• 金额: $ 3万
• 依托单位: Sojo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20560732/
• 关键词: リポソーム(複合脂質膜); がん化学療法; 糖; 脂質; アポトーシス; 細胞膜

Hybrid liposomes (HL) can be prepared by sonication of vesicular and micellar molecules in a buffer solution. The physical properties of HL such as size, shape, membrane fluidity, and the temperature of phase separation can be controlled by changing the constituents and compositional ratio. We have employed three-component hybrid liposomes (THL) composed of L-α-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene sorbitane monolaurate (Tween 20), and decyl-β-lactopranoside (LactC_<10>) (DMPC : Tween 20 : LactC_<10> = 65 : 7 : 28) for the research of cancers and interesting results are as follows;(A) LactC_<10> was successfully produced from decyl-β-lactoheptaacetate (Ac-LactC_<10>), which was obtained by glycosylation and deacetylation of β-lactoseoctaacetate (Ac-Lact).(B) IC50 values of three-component hybrid liposomes including lactose surfactants (HL/LactC_<10>) for human hepatoma (Hep-G2, Huh-7) cells were smaller than those of two-component hybrid liposomes (HL).(C) The thickness of fixed aqueous layer (TFAL) of HL/LactC_<10> was about twice that of HL. The result suggests that the inhibitory effects of HL/LactC_<10> could be related to hydration in tumor cells.(D) Induction of apoptosis by HL/LactC_<10> for Hep-G2 and Huh-7 cells were obtained.Thus, this study demonstrated the importance of relation between hydration and inhibition of the growth of hepatoma cells using THL having specific fixed aqueous layers.

杂化脂质体（HL）可以通过在缓冲溶液中对囊泡和胶束分子进行超声处理来制备。通过改变HL的组分和组成比，可以控制HL的尺寸、形状、膜流动性和相分离温度等物理性质。本研究采用L-α-二myristoylphosphatidycholine （DMPC）、聚氧乙烯山梨糖烷单月果酸酯（Tween 20）和癸基-β-乳糖苷（LactC_<10>） （DMPC: Tween 20: LactC_<10> = 65:7: 28）组成的三组分杂交脂质体（THL）研究癌症，有趣的结果如下：(A)以β-乳糖八乙酸酯（Ac-LactC_<10>）为原料，经糖基化和去乙酰化反应制得decyl-β-乳七乙酸酯（ac - lacc_ <10>）。(B)含乳糖表面活性剂的三组分杂交脂质体（HL/LactC_<10>）对人肝癌（Hep-G2, Huh-7）细胞的IC50值小于双组分杂交脂质体（HL）。(C) HL/LactC_<10>的固定水层厚度（tal）约为HL的2倍。结果提示HL/LactC_<10>的抑制作用可能与肿瘤细胞的水合作用有关。(D) HL/LactC_<10>诱导Hep-G2和Huh-7细胞凋亡。因此，本研究证明了水合作用与使用具有特定固定水层的THL抑制肝癌细胞生长之间关系的重要性。

项目成果

Inhibitory Effects of Cationic Hybrid Liposomes on the Growth of Human Renal Cell Carcinoma

阳离子杂化脂质体对人肾细胞癌生长的抑制作用

• 发表时间: 2010
• 期刊: Anticancer Research 30
• 作者: 山下弘巳;桑原泰隆;大道徹太郎;亀川孝;森浩亮;Yusuke Mikami ; Kaori Ebata ; TakatoMitsudome ; Tomoo Mizugaki ; KoichiroJitsukawa ; Kiyotomi Kaneda;Masayo Umebayashi
• 通讯作者: Masayo Umebayashi

腎臓がんに対するカチオニックハイブリッドリポソームのアポトーシス誘導

阳离子杂化脂质体诱导肾癌细胞凋亡

• 发表时间: 2010
• 作者: 梶本武志;畑俊充;田川雅人;小嶋浩嗣;日野元貴
• 通讯作者: 日野元貴

蛍光ハイブリッドリポソームのがん診断薬への応用

荧光杂化脂质体在癌症诊断药物中的应用

• 发表时间: 2010
• 作者: 松本康司;竹野貴法;三木寛之;行原真美子
• 通讯作者: 行原真美子

Membrane Targeted Nanothrapy with Hybrid Liposomes for Colon Tumor Cells Leading to Apoptosis

混合脂质体膜靶向纳米疗法治疗结肠肿瘤细胞导致细胞凋亡

• 发表时间: 2009
• 作者: Noujima;A.; Mikami;Y.; Mitsudome;T.; Mizugaki;T.; Jitsukawa;K.; Kaneda;K.;Yuji Komizu
• 通讯作者: Yuji Komizu

Inhibitory Effects of Cationic Hybrid Liposomes on the Growth of Renal Tumors along with Apoptosis

阳离子杂化脂质体对肾肿瘤生长及细胞凋亡的抑制作用

• 发表时间: 2009
• 作者: 岸田和博;横田久美子;岡本昭夫;田川雅人;Shinsuke Takeuchi;Masayo Umebayashi
• 通讯作者: Masayo Umebayashi