Specific Inhibitory Effects of Hybrid Liposomes on the Growth of Various Tumor Cells

1.混合脂质体对肿瘤细胞在体外生长的抑制作用：混合脂质体由脂质体组成的一种不同的头部组（双离子L-α-二聚氰酸酯胆碱（DMPC）、阴离子L α-二聚氰酸酯glycerol （DMPG）和cationic 1），2-dimyristoyl-3-trimethylammonium propane （DMTAP）和polyoxyethylene （10）十二烷基醚（C-D212-D2（EO）-D210-D2） on the growth of tumor cells （human lung carcinoma （RERF-LC-OK）、human hepatoma （Hep-G2）、human stomach tumor （GT3TKB） in vitro were examined。It is attractive that the highly specific inhibitory effect of the hybrid liposomes composed of DMPG/10 mol % （C-D212-D2（EO）-D210-D2） On the growth of RERF-LC-OK and GT3TKB was obtained without any antitumor drugs.2。Apoptosis Hybrid脂质体的诱导我们研究了由dimyristoylphosphatidylcholine和polyoxyethylenealkyl ether进行的抑制作用，使肿瘤细胞的生长与Apoptosis有关。通过HL-60细胞中混合脂质体的凋亡定义，对琼脂糖凝胶电泳的基础进行了验证，DNA和流式细胞分析用前列腺碘化物固定方法进行了分析。一个荧光显微图使用荧光素异硫氰酸酯作为荧光探测显示，混合脂质体应该与肿瘤细胞膜一起失效。在融合后，混合脂质体的累积被揭示为肿瘤细胞膜的基础。用混合脂质体的黑色素瘤小鼠小鼠模型的化学疗法已由Lewis lung carcinoma细胞的内部炎症研究所证实。可显著延长的存活率（480%）被管理成老鼠的混合脂质体。以下结果建议是，混合储存液体应该对癌症的治疗产生影响。长期而言，混合脂质体没有毒性，在正常老鼠体内发现了没有副作用的情况。

1. Inhibitory Effects of Hybrid Liposomes on the Growth of tumor Cells in vitroThe inhibitory effects of hybrid liposomes composed of lipids having a variety of head groups (zwitterionic L-α-dimyristoylphosphatidylcholine (DMPC), anionic L α-dimyristoylphosphatidyl glycerol (DMPG) and cationic 1, 2-dimyristoyl-3-trimethylammonium propane (DMTAP)) and polyoxyethylene (10) dodecyl ether (CィイD212ィエD2(EO)ィイD210ィエD2) on the growth of tumor cells (human lung carcinoma (RERF-LC-OK), human hepatoma (Hep-G2), human stomach tumor (GT3TKB)) in vitro were examined. It is attractive that the highly specific inhibitory effect of the hybrid liposomes composed of DMPG/10mol% (CィイD212ィエD2(EO)ィイD210ィエD2) On the growth of RERF-LC-OK and GT3TKB was obtained without any antitumor drugs.2. Induction of Apoptosis Hybrid LiposomesWe examined antitumor mechanism of the hybrid liposomes composed of dimyristoylphosphatidylcholine and polyoxyethylenealkyl ether having inhibitory action toward the growth of tumor cells related to apoptosis. The induction of apoptosis by the hybrid liposomes in HL-60 cells was verified on the basis of agarose gel electrophoresis of DNA and flow cytometry analysis with the propidium iodide staining method. A fluorescence micrograph taken using fluorescein isothiocyanate as a fluorescence probe showed that the hybrid liposomes should fuse with the tumor cell membrane. After fusion, accumulation of the hybrid liposomes to tumor cell membrane was revealed on the basis of DNA analysis.3. Chemotherapy with Hybrid Liposomes for MelanomatosisA mouse model of lung carcinoma was established by intraperitoneal inoculate of Lewis lung carcinoma cells. The remarkably prolonged survival (480%) was attained by administering the hybrid liposomes to mice. This result suggests that the hybrid liposomes should be effective for the treatment of cancers. Furthermore, no toxicity of the hybrid liposomes was obtained in normal rats in vivo with no side effects.