Study of the intestinal epithelial stem cell niche using transgenic frogs

利用转基因青蛙研究肠上皮干细胞生态位

• 批准号: 20570060
• 负责人: OKA Atsuko
• 金额: $ 3.08万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570060/
• 关键词: トランスジェニックカエル; 甲状腺ホルモン受容体; 小腸; 器官培養; 組織間相互作用; 幹細胞; 変態; 脱分化; 甲状腺ホルモン応答遺伝子; シグナル伝達経路; 上皮再生; ニッチ

In the amphibian digestive tract, thyroid hormone(TH) induces stem cells that generate the adult epithelium similar to the mammalian counterpart. In the present study, to clarify molecular mechanisms regulating the stem cells, we performed tissue recombinant culture experiments, using the small intestine of transgenic Xenopus laevis tadpoles. Our results indicate that both TH response genes expressed in the epithelium and those in the connective tissue are necessary for the larval epithelium to dedifferentiate into the adult stem cells. In addition, expression analyses of TH response genes suggest important roles of Shh and Wnt signaling pathways in establishment of the stem cell niche.

在两栖动物消化道中，甲状腺激素（TH）诱导干细胞产生与哺乳动物相似的成体上皮。本研究利用转基因非洲爪蟾蝌蚪的小肠进行了组织重组培养实验，以阐明干细胞调控的分子机制。我们的研究结果表明，在上皮和结缔组织中表达的TH应答基因都是幼虫上皮向成体干细胞去分化所必需的。此外，TH应答基因的表达分析表明Shh和Wnt信号通路在干细胞生态位的建立中起重要作用。

项目成果

Tissue-dependent induction of apoptosis by matrix metalloproteinase stromelysin-3 during amphibian metamorphosis.

• DOI: 10.1002/bdrc.20170
• 发表时间: 2010-03
• 期刊: BIRTH DEFECTS RESEARCH PART C-EMBRYO TODAY-REVIEWS
• 作者: Mathew, Smita;Fu, Liezhen;Hasebe, Takashi;Ishizuya-Oka, Atsuko;Shi, Yun-Bo
• 通讯作者: Shi, Yun-Bo

Spatio-temporal expression profile of stem cell-associated gene LGR5 in the intestine during thyroid hormone-dependent metamorphosis in Xenopus laevis.

• DOI: 10.1371/journal.pone.0013605
• 发表时间: 2010-10-22
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Sun G;Hasebe T;Fujimoto K;Lu R;Fu L;Matsuda H;Kajita M;Ishizuya-Oka A;Shi YB
• 通讯作者: Shi YB

Evolutionary insights into postembryonic development of adult intestinal stem cells.

• DOI: 10.1186/2045-3701-1-37
• 发表时间: 2011-11-16
• 期刊: Cell & bioscience
• 影响因子: 7.5
• 作者: Ishizuya-Oka A;Shi YB
• 通讯作者: Shi YB

Thyroid hormone-upregulated hedgehog interacting protein is involved in larval-to-adult intestinal remodeling by regulating sonic hedgehog signaling pathway in Xenopus Iaevis.

甲状腺激素上调的 Hedgehog 相互作用蛋白通过调节非洲爪蟾的声波 Hedgehog 信号通路参与幼虫到成虫的肠道重塑。

• 发表时间: 2008
• 期刊: Dev.Dyn.(有) 237
• 作者: Hasebe T.;Kajita M.;Shi Y.-B.;Ishizuya-Oka A.
• 通讯作者: Ishizuya-Oka A.

ドミナントポジティブ型甲状腺ホルモン受容体の発現により誘起されるアフリカツメガエル成体型腸上皮の発生

显性阳性甲状腺激素受体表达诱导非洲爪蟾成体肠上皮的发育

• 发表时间: 2010
• 作者: 長谷部孝;岡敦子
• 通讯作者: 岡敦子