Analysis of AVR-Pia gene of trice blast fungus and its application for the pathogenicity differentiation

稻瘟病菌AVR-Pia基因分析及其在致病性鉴别中的应用

• 批准号: 20580043
• 负责人: SONE Teruo
• 金额: $ 3万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580043/
• 关键词: いもち病菌; Magnaporthe oryzae; PCR; イネ; 非病原性遺伝子; エフェクター; 突然変異; 病原性

Successful molecular cloning and identification of AVR-Pia avirulence gene from Magnaporthe oryzae was demonstrated in this study. AVR-Pia gene was located at the same position as the locus which was genetically analyzed using Chinese isolates. AVR-Pia was expressed only during infection, and the product was found to be secreted into compatible host cells, through BICs of the infection hyphae. Mechanism of gene deletion in the mutant Ina168m95-1 was elucidated to be the homologous recombination between two adjacent copies of the DNA transposon Occan. PCR amplification of the gene is effective way to determine the pathogenicity toward rice cultivar with Pia R gene.

本研究成功克隆并鉴定了稻瘟病菌AVR-Pia无毒基因。AVR-Pia基因与中国分离株基因定位结果一致。AVR-Pia仅在感染期间表达，并且发现该产物通过感染菌丝的BIC分泌到相容的宿主细胞中。突变体Ina 168 m95 -1的基因缺失机制被阐明为两个相邻拷贝的DNA转座子Occan之间的同源重组。PCR扩增Pia R基因是鉴定Pia R基因对水稻品种致病性的有效方法。

项目成果

イネいもち病菌の非病原性遺伝子AVR-Piaの発現時期の解析

稻瘟病菌无毒基因AVR-Pia的表达时机分析

• 发表时间: 2010
• 作者: 三木慎介;ら
• 通讯作者: ら

Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae

• DOI: 10.1111/j.1364-3703.2009.00534.x
• 发表时间: 2009-05-01
• 期刊: MOLECULAR PLANT PATHOLOGY
• 影响因子: 4.9
• 作者: Miki, Shinsuke;Matsui, Kotaro;Sone, Teruo
• 通讯作者: Sone, Teruo

イネいもち病菌の非病原性遺伝子AVR-Piaのin planta発現解析

稻瘟病菌无毒基因AVR-Pia在植物体内的表达分析

• 发表时间: 2011
• 作者: 三木慎介;ら
• 通讯作者: ら

イネいもち病菌の非病原性遺伝子AVR-Pia遺伝子座の解析

稻瘟病菌无毒基因AVR-Pia位点分析

• 发表时间: 2009
• 作者: Sone;T;三木慎介・佐藤祐樹・大塚圭輔・芦澤斌人・平八重一之・曾根輝雄;大塚圭輔・三木慎介・安田伸子・藤田佳克・曾根輝雄
• 通讯作者: 大塚圭輔・三木慎介・安田伸子・藤田佳克・曾根輝雄

イネいもち病菌のAVR-Pia遺伝子のクローニング

稻瘟菌AVR-Pia基因的克隆

• 发表时间: 2009
• 作者: 三木慎介;ら
• 通讯作者: ら