Analysis of AVR-Pia gene of trice blast fungus and its application for the pathogenicity differentiation

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Successful molecular cloning and identification of AVR-Pia avirulence gene from Magnaporthe oryzae was demonstrated in this study. AVR-Pia gene was located at the same position as the locus which was genetically analyzed using Chinese isolates. AVR-Pia was expressed only during infection, and the product was found to be secreted into compatible host cells, through BICs of the infection hyphae. Mechanism of gene deletion in the mutant Ina168m95-1 was elucidated to be the homologous recombination between two adjacent copies of the DNA transposon Occan. PCR amplification of the gene is effective way to determine the pathogenicity toward rice cultivar with Pia R gene.