Studies on the physiological functions of phospholipases A2

磷脂酶A2的生理功能研究

• 批准号: 20580074
• 负责人: ARIOKA Manabu
• 金额: $ 3.08万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580074/
• 关键词: 微生物代謝; ホスホリパーゼA2; リゾリン脂質; MAPキナーゼ; ミトコンドリア; 麹菌; PC12

In PC12 cells, nerve growth factor(NGF) activates MAPK phosphorylation and induces neuronal differentiation, which is potently enhanced by lysophosphatidylcholine(LPC). No other lysophospholipids tested displayed similar activity. MAPK activation/phosphorylation that occurs downstream of other growth factors, such as epidermal and fibroblast growth factors, was not affected by LPC. TrkA autophosphorylation elicited by NGF was markedly enhanced by LPC, suggesting that the effect of LPC was specific to NGF-TrkA signaling pathway. AoPlaA in the filamentous fungus Aspergillus oryzae localizes to mitochondria. Subcellular fractionation experiment showed that AoPlaA localized to the intramembrane space of mitochondria. Phospholipid analysis demonstrated that phosphatidylethanolamine and cardiolipin was decreased and increased in AoplaA-overexpressing and-deleted strains, respectively.

在PC 12细胞中，神经生长因子（NGF）激活MAPK磷酸化并诱导神经元分化，这被溶血磷脂酰胆碱（LPC）有力地增强。没有其他测试的溶血磷脂显示出类似的活性。MAPK活化/磷酸化发生在其他生长因子的下游，如表皮和成纤维细胞生长因子，不受LPC的影响。LPC能显著增强NGF诱导的TrkA自磷酸化，提示LPC的作用是NGF-TrkA信号通路特异性的。丝状真菌AspergillusplaA定位于线粒体。亚细胞分级分离实验表明AoPlaA定位于线粒体的膜内空间。磷脂分析表明，磷脂酰乙醇胺和心磷脂减少和增加AoplaA过表达和删除菌株，分别。

项目成果

Distinct enzymatic and cellular characteristics of two secretory phospholipases A2 in the filamentous fungus Aspergillus oryzae

• DOI: 10.1016/j.fgb.2009.12.011
• 发表时间: 2010-04-01
• 期刊: FUNGAL GENETICS AND BIOLOGY
• 影响因子: 3
• 作者: Nakahama, Tomoyuki;Nakanishi, Yoshito;Arioka, Manabu
• 通讯作者: Arioka, Manabu

Enhanced neurotrophic effect of nerve growth factor by lysophosphatidylcholine

溶血磷脂酰胆碱增强神经生长因子的神经营养作用

• 发表时间: 2009
• 作者: Itakura;A.;Matsuki;Y.;and Arioka;M.
• 通讯作者: M.

麹菌 A.oryzae における細胞質型ホスホリパーゼA_2の局在と機能の解析

米曲霉胞质磷脂酶A_2的定位及功能分析

• 发表时间: 2008
• 作者: 高谷康平;北本勝ひこ;有岡学
• 通讯作者: 有岡学

麹菌A. oryzaeにおける細胞質型ホスホリパーゼA2の局在と機能の解析

米曲霉胞质磷脂酶A2的定位及功能分析

• 发表时间: 2008
• 作者: 高谷康平;北本勝ひこ;有岡学
• 通讯作者: 有岡学

A cytosolic phospholipase A2-like protein in the filamentous fungus Aspergillus oryzae localizes to the intramembrane space of the mitochondria.

• DOI: 10.1111/j.1574-6968.2009.01818.x
• 发表时间: 2009-12
• 期刊: FEMS microbiology letters
• 影响因子: 2.1
• 作者: Kohei Takaya;Yujiro Higuchi;K. Kitamoto;M. Arioka