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Development of reagentless sensing system for the determination of antioxidative activity using micro-distance between the electrodes

开发无试剂传感系统，利用电极之间的微距离测定抗氧化活性

基本信息

批准号：
20580144
负责人：
OKUMA Hirokazu
金额：
$ 2.91万
依托单位：
Toyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580144/
关键词：
抗酸化活性抗酸化能活性酸素スクリーン印刷電極

项目摘要

Antioxidative activity is an important factor in the development of high-functional food products utilizing such characteristic of foods, and measurement of the activity is significant. This study was performed aiming at development of an antioxidative activity measurement method using no enzyme or specific reagent. When a voltage of 0.33 V is applied to an electrode such as platinum in aqueous solution, O_2^- is generated on the electrode, and the generated O_2^- produces H_2O_2 by disproportional reaction. In this study, using this principle, a negative voltage was applied to the electrode to generate O_2^-, and H_2O_2 was produced by disproportional reaction. When an antioxidative substance is present, the substance captures O_2^- and decreases the production of H_2O_2 by disproportional reaction. Detection of the amount of H_2O_2 using a proximal electrode allows investigation of antioxidative activity of substances and measurement of the activity.To effectively detect H_2O_2 produced by disproportional reaction of O_2^-, a screen-printed carbon ink electrode (W1) for measurement of H_2O_2 was placed on the opposite side of the screen-printed carbon ink electrode (W2) for generation of O_2^-. For the counter and reference electrodes, screen-printed carbon ink and silver-silver chloride ink electrode were used, respectively. To generate O_2^-, -0.9 V was applied to the W2 electrode in KCl. To the W1 electrode, 1.1 V was applied to confirm the sensor response of this electrode. The distance between the electrodes was set to 240μm. O_2^--scavenging activity of catechin standard sample solutions, tea and vegetables were measured employing this system.The results for teas and vegetables correlated satisfactorily with those obtained DPPH method. These results suggest that the proposed system provides a simple and rapid method for the determination of antioxidative activity.

在利用食品的这种特性开发高功能食品的过程中，抗氧化活性是一个重要因素，其活性的测量具有重要意义。本研究旨在开发一种不使用酶或特定试剂的抗氧化活性测定方法。在0.33V的电压下，铂等电极在水溶液中产生O_2^-，生成的O_2^-通过不成比例反应生成H_2O_2。在本研究中，利用这一原理，在电极上施加负电压产生O2^-，通过不成比例反应生成H_2O_2。当存在抗氧化剂时，该物质捕获O_2^-并通过不成比例反应减少H_2O_2的产生。使用近端电极检测H_2O_2的量可以研究物质的抗氧化活性并测量其活性。为了有效地检测O_2^-不成比例反应产生的H_2O_2，将用于测量H_2O_2的丝网印刷碳墨电极(W1)放置在用于产生O_2^-的丝网印刷碳墨电极(W2)的对侧。对电极和参比电极分别采用丝网印刷碳墨和氯化银-氯化银墨水电极。为了产生O2^-，在KCl中对W2电极施加-0.9V。对于W1电极，施加1.1V以确认该电极的传感器响应。利用该系统对儿茶素标准样品溶液、茶叶和蔬菜的清除活性进行了测定，对茶叶和蔬菜的测定结果与DPPH法测定结果一致，结果令人满意。电极间距为240DPPH/μ。这些结果表明，该体系为抗氧化活性的测定提供了一种简单、快速的方法。