Research for host-recognition and responses against endotoxin modified by pathogenic bacteria.

宿主识别和针对病原菌修饰的内毒素的反应的研究。

• 批准号: 20590073
• 负责人: KAWASAKI Kiyoshi
• 金额: $ 3.08万
• 依托单位: Doshisha Women's College of Liberal Arts
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590073/
• 关键词: エンドトキシン; Toll-like receptor; 細菌; 外膜; 潜伏化; 脱アシル化; リポ多糖; 活性調節; 膜透過性; 脂質; シグナル伝達; 自然免疫

Regulatory machineries of lipid A modification enzymes were analyzed in this study. Lipid A modification enzyme PagL is usually latent, and the property of PagL to associate with lipopolysaccharide was shown to be important for the latency. Furthermore, the biological significance of the PagL latency was examined. PagL latency was shown to be beneficial for bacteria to produce effective membrane permeation barrier.

本研究分析了类脂A修饰酶的调控机制。脂质A修饰酶PagL通常是潜伏的，并且PagL与脂多糖缔合的性质被证明对于潜伏是重要的。此外，PagL潜伏期的生物学意义进行了检查。PagL潜伏期被证明有利于细菌产生有效的膜渗透屏障。

项目成果

Extracellular loops of lipid A 3-Ο-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonell a enterica serovar Typhimurium.

脂质 A 3-O-脱酰酶 PagL 的胞外环参与识别鼠伤寒沙门氏菌中基于氨基阿拉伯糖的膜修饰。

• 发表时间: 2008
• 期刊: J. Bacteriol. 190
• 作者: T. Manabe;他1名
• 通讯作者: 他1名

Mutations in the lipid A deacylase PagL which release the enzyme from its latency affect the ability of PagL to interact with lipopolysaccharide in Salmonella enterica serovar Typhimurium

脂质 A 脱酰酶 PagL 中的突变从潜伏期释放该酶，影响 PagL 与鼠伤寒沙门氏菌中脂多糖相互作用的能力

• 发表时间: 2010
• 期刊: Biochem.Biophys.Res.Commun.
• 作者: Manabe T.;他2名
• 通讯作者: 他2名

Exacraoellular loops of lipid A 3-O-deacylase PagL are involved in recognit ionof aminoarabinose-based membrane modifications in Salmonella enterica

脂质 A 3-O-脱酰酶 PagL 的胞外环参与肠沙门氏菌中基于氨基阿拉伯糖的膜修饰的识别

• 发表时间: 2008
• 作者: Kiyoshi Kawasaki;他1名
• 通讯作者: 他1名

The preparation of a lipidic endoxon in affects its biological activities

脂质内氧蛋白的制备影响其生物活性

• 发表时间: 2008
• 期刊: Biol. Pharm. Bull. 31
• 作者: Kazuo Okemoto;他3名
• 通讯作者: 他3名

Salmonella enterica serovar Typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages

• DOI: 10.1016/j.febslet.2009.11.062
• 发表时间: 2010-01-04
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Kawano, Miyuki;Manabe, Takayuki;Kawasaki, Kiyoshi
• 通讯作者: Kawasaki, Kiyoshi