Comparative analysis of micro RNA expressions on between reactive lymphoid follicles and follicular lymphomas focusing to post-translational process.

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We planned the expression analysis of micro RNAs on between human follicular lymphomas (FLs) and their non-neoplastic counterpart, germinal centers (GCs) to elucidate the lymphomagenesis of FLs. From the results of miRNA profiling of human FLs in comparison with that of GCs, also of quantitative analysis by real-time PCR for sorted GC cells and FL cells, miR-146a and miR-193b revealed to be up-regulated in FLs than GCs, whereas miR-26a seemed to be down-regulated. Based on these results, we further generated transgenic mice overexpressing mouse miR-146a under regulation by immunoglobulin enhancer/promotor, and explore the phenotypic features of their B cells. Consequently, splenic GCs in miR-146a Tg mice seemed to be enlarged in response to stimulation by an exogenous antigen than those in wild type mice. The details are still under investigation now.