The role of micro RNA and methylated gene in multistep genesis and prognosis of multiple myeloma

微小RNA和甲基化基因在多发性骨髓瘤多步发生和预后中的作用

• 批准号: 20590556
• 负责人: HANDA Hiroshi
• 金额: $ 3.08万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590556/
• 关键词: 骨髄腫; 多段階発癌; メチル化遺伝子; microRNA; micro RNA; 予後

We detected methylated WWOX promoter in myeloma cell lines, myeloma patients but not in MGUS patients. The aberrant transcript of WWOX mRNA was detected in myeloma patients, MGUS and cell lines. The expression level of micro-RNA miR15a 15b, 16 were very low or lacked in purified plasma cells in some patients. MiR 15a, 15b, 16 levels were lower in plasma cells of MM than those of MGUS. On the contrary, Dnmts and Mcl-1 levels were higher in plasma cells of MM than those of MGUS.

我们在骨髓瘤细胞系、骨髓瘤患者中检测到WWOX甲基化启动子，但在MGUS患者中未检测到。在骨髓瘤患者、MGUS和细胞系中均检测到WWOX mRNA的异常转录。部分患者的纯化浆细胞中microRNA miR15a、15b、16的表达水平很低或缺失。MM患者浆细胞中MIR 15a、15b、16表达水平低于MGUS患者。MM患者浆细胞中DNMTs和Mcl-1水平明显高于MGUS患者。

项目成果

Common fragile site gene FHIT (fragile histidine triad gene) and WWOX (WW domain containing oxidoreductase) expression are frequently altered in multiple myeloma.

常见脆弱位点基因 FHIT（脆弱组氨酸三联体基因）和 WWOX（含氧化还原酶的 WW 结构域）表达在多发性骨髓瘤中经常发生改变。

• 发表时间: 2011
• 作者: Yuki Naito;Yutaka Yoshikawa;Hiroyuki Yasui;半田寛
• 通讯作者: 半田寛