The role of micro RNA and methylated gene in multistep genesis and prognosis of multiple myeloma

我们在骨髓瘤细胞系、骨髓瘤患者中检测到WWOX甲基化启动子，但在MGUS患者中未检测到。在骨髓瘤患者、MGUS和细胞系中均检测到WWOX mRNA的异常转录。部分患者的纯化浆细胞中microRNA miR15a、15b、16的表达水平很低或缺失。MM患者浆细胞中MIR 15a、15b、16表达水平低于MGUS患者。MM患者浆细胞中DNMTs和Mcl-1水平明显高于MGUS患者。

We detected methylated WWOX promoter in myeloma cell lines, myeloma patients but not in MGUS patients. The aberrant transcript of WWOX mRNA was detected in myeloma patients, MGUS and cell lines. The expression level of micro-RNA miR15a 15b, 16 were very low or lacked in purified plasma cells in some patients. MiR 15a, 15b, 16 levels were lower in plasma cells of MM than those of MGUS. On the contrary, Dnmts and Mcl-1 levels were higher in plasma cells of MM than those of MGUS.