Comprehensive gene expression analysis of cancer-infiltrating lymphocytes and peripheral blood cells for elucidation of cancer immunity kinesis

使用显示增殖期和随后的熄灭期的小鼠皮下肝细胞瘤模型，对局部癌浸润炎性细胞以及外周血细胞的基因表达谱进行分析。外周血的基因表达谱的特征根据癌细胞注射后的天数划分的组而不同。局部癌症浸润性炎性细胞以及具有最大尺寸水平肿瘤的小鼠的外周血细胞的基因表达的共同特征显示IL-6信号和对未折叠蛋白的反应。至于基因表达的个别特征过程，干扰素信号传导、NK细胞的细胞毒性、局部癌症浸润炎性细胞的补体系统以及外周血的IL-10抗炎反应、白介素信号传导分别具有特征。综合分析肿瘤局部炎症反应和外周血细胞的基因表达，可以揭示局部和全身炎症反应的共同特征和区别。

Using murine subcutaneous hapatoma model which shows the proliferating and succeeding extinguishing phases, analysis of gene expression profile for local cancer-infiltrating inflammatory cells as well as peripheral blood cells was performed. The features of gene expression profiles for peripheral blood were distinct depending on the groups divided by the days after cancer cells injection. The common feature of gene expression of local cancer-infiltrating inflammatory cells as well as peripheral blood cells for mice with the tumors at the maximum size levels showed IL-6 signal and response to unfoleded proteins. As for the individually characteristic processes of gene expression, interferon signaling, NK cell cytotoxicity, complement system for local cancer-infiltrating inflammatory cells, and IL-10 anti-inflammatory response, amphoterin signaling for peripheral blood, were featured, respectively. These suggest that comprehensive analysis of gene expression of local cancer inflammatory response and peripheral blood cells will reveal the common as well as the distinguished features of local and systemic inflammatory responses.