Comprehensive gene expression analysis of cancer-infiltrating lymphocytes and peripheral blood cells for elucidation of cancer immunity kinesis

采用小鼠皮下单纯性虹膜瘤模型，分析了肿瘤浸润炎性细胞和外周血细胞的基因表达谱。外周血基因表达谱的特征是不同的，这取决于各组注射癌细胞后的天数。肿瘤最大大小水平小鼠的局部癌浸润炎性细胞和外周血细胞基因表达的共同特征是IL-6信号和对未折叠蛋白的反应。在基因表达的个体特征过程中，干扰素信号传导、NK细胞细胞毒性、局部肿瘤浸润炎性细胞补体系统和IL-10抗炎反应、外周血两性激素信号传导分别得到了表征。这些提示，综合分析局部肿瘤炎症反应和外周血细胞的基因表达将揭示局部和全身炎症反应的共同特征和各自的特点。

Using murine subcutaneous hapatoma model which shows the proliferating and succeeding extinguishing phases, analysis of gene expression profile for local cancer-infiltrating inflammatory cells as well as peripheral blood cells was performed. The features of gene expression profiles for peripheral blood were distinct depending on the groups divided by the days after cancer cells injection. The common feature of gene expression of local cancer-infiltrating inflammatory cells as well as peripheral blood cells for mice with the tumors at the maximum size levels showed IL-6 signal and response to unfoleded proteins. As for the individually characteristic processes of gene expression, interferon signaling, NK cell cytotoxicity, complement system for local cancer-infiltrating inflammatory cells, and IL-10 anti-inflammatory response, amphoterin signaling for peripheral blood, were featured, respectively. These suggest that comprehensive analysis of gene expression of local cancer inflammatory response and peripheral blood cells will reveal the common as well as the distinguished features of local and systemic inflammatory responses.