Gene transfer programming of mesenchymal stem cells for repairing and regeneration of the liver, and its application for liver regeneration therapy

间充质干细胞肝脏修复再生基因转移编程及其在肝脏再生治疗中的应用

• 批准号: 23590968
• 负责人: SAKAI Yoshio
• 金额: $ 3.24万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590968/
• 关键词: 肝臓学; 肝再生; 間葉系幹細胞; 遺伝子導入; エレクトロポレーション

The experimental trial of differentiation of adipose-tissue derived stromal cells to hepatocyte by means of gene transduction was conducted. The pCMV6-HNF4a-GFP expressing GFP-tagged HNF4a and the pCMV6-HNF1b-GFP expressing GFP-tagged HNF1b was transduced to adipose tissue-derived stromal cells by electroporation method. Cells at day 15 after transduction was assessed for gene expression by real-time detective PCR. Cells transduced with pCMV6-HNF4a-GFP expressed HNF4a, however, did not express albumin. Cells transduced with pCMV6-HNF1b-GFP neither expressed HNF1b nor albumin. Improvement is required for differentiation of adipose tissue derived stromal cells by electroporation method.

采用基因转导的方法，对脂肪基质细胞向肝细胞分化进行了实验研究。通过电穿孔法将表达GFP标记的HNF 4a的pCMV 6-HNF 4a-GFP和表达GFP标记的HNF 1b的pCMV 6-HNF 1b-GFP转导至脂肪组织来源的基质细胞。通过实时检测PCR评估转导后第15天的细胞的基因表达。用pCMV 6-HNF 4a-GFP转导的细胞表达HNF 4a，然而，不表达白蛋白。用pCMV 6-HNF 1b-GFP转导的细胞既不表达HNF 1b也不表达白蛋白。电穿孔法诱导脂肪基质细胞分化的方法有待改进。

项目成果

Therapeutic effect of adipose derived mesenchymal stem cell to murine NASH liver cirrhosis inactivating Kupffer/Macrophage and helper T cell

脂肪间充质干细胞对小鼠NASH肝硬化灭活Kupffer/巨噬细胞和辅助T细胞的治疗作用

• 发表时间: 2012
• 作者: Akihiro Seki;Yoshio Sakai;Takuya Komura;Mami Higashimoto;Haruo Fujinaga;Masao Honda,Takashi Wada;Toshinari Takamura;Takahiro Ochiya;Shuichi Kaneko
• 通讯作者: Shuichi Kaneko

Features of immunomodulatory and therapeutic effects of mesenchymal stromal/stem cells on non-alcoholic steatohepatitis murine model in early and cirrhotic phases.

间充质基质/干细胞对早期和肝硬化小鼠非酒精性脂肪性肝炎模型的免疫调节和治疗作用特征。

• 作者: Akihiro Seki;Yoshio Sakai;Mami Higashimoto;Takuya Komura;Alessandro Nasti;Keiko Yoshida;Takahiro Ochiya;Takashi Wada;Masao Honda;Shuichi Kaneko.
• 通讯作者: Shuichi Kaneko.

肪組織由来間質細胞を用いた肝硬変に対する肝再生療法実用化への基礎的検討

利用脂肪组织源性基质细胞进行肝再生治疗肝硬化的实际应用基础研究

• 作者: 酒井佳夫;関晃裕;金子周一.
• 通讯作者: 金子周一.

Association of changes in the gene expression profile of blood cells with the local tumor inflammatory response in a murine tumor model

• DOI: 10.1016/j.bbrc.2012.10.004
• 发表时间: 2012-11-09
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Sakai, Yoshio;Tatsumi, Isamu;Kaneko, Shuichi
• 通讯作者: Kaneko, Shuichi

Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma

单核细胞趋化蛋白-1 的膜结合形式增强自杀基因治疗在肝细胞癌模型中的抗肿瘤作用

• 发表时间: 2012
• 期刊: Cancer Gene Ther
• 影响因子: 6.4
• 作者: Marukawa Y;Nakamoto Y;Kakinoki K;Tsuchiyama T;Iida N;Kagaya T;Sakai Y;Naito M;Mukaida N;Kaneko S
• 通讯作者: Kaneko S