Gene transfer programming of mesenchymal stem cells for repairing and regeneration of the liver, and its application for liver regeneration therapy

用基因转导的方法进行脂肪基质细胞向肝细胞分化的实验研究。用电穿孔法将表达GFP标记的HNF4a的pCMV6-HNF4a-GFP和表达GFP标记的HNF1B的pCMV6-HNF1B-GFP分别导入脂肪组织来源的基质细胞。用实时定量聚合酶链式反应检测转导后第15天的细胞基因表达。转导pCMV6-HNF4a-GFP的细胞表达HNF4a，但不表达白蛋白。转导pCMV6-HNF1B-GFP的细胞既不表达HNF1B，也不表达白蛋白。电穿孔法诱导脂肪组织来源的基质细胞分化尚需改进。

The experimental trial of differentiation of adipose-tissue derived stromal cells to hepatocyte by means of gene transduction was conducted. The pCMV6-HNF4a-GFP expressing GFP-tagged HNF4a and the pCMV6-HNF1b-GFP expressing GFP-tagged HNF1b was transduced to adipose tissue-derived stromal cells by electroporation method. Cells at day 15 after transduction was assessed for gene expression by real-time detective PCR. Cells transduced with pCMV6-HNF4a-GFP expressed HNF4a, however, did not express albumin. Cells transduced with pCMV6-HNF1b-GFP neither expressed HNF1b nor albumin. Improvement is required for differentiation of adipose tissue derived stromal cells by electroporation method.