Research of endothelial progenitor cell-capture STENT

内皮祖细胞捕获支架的研究

• 批准号: 20590819
• 负责人: YAMAGISHI Masakazu
• 金额: $ 3万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590819/
• 关键词: 臨床心血管病態学; 血管内皮前駆細胞; ステント開発; 血管内皮成長因子

The positive cell of human endothelial progenitor cell (EPS) markers were isolated from peripheral blood mononuclear cell and were analyzed mRNA level by real time RT-PCR. There were few KDR positive cells into the human mononuclear cell. Endothelial colony formation cells (early EPS) colonies were identified on day 14 to 21 after it were cultured. We assessed immunophenotyping of early EPC using fluorescence microscope and characterized the early EPS by non quantitative RT-PCR. We established method of experiment for identification of EPSs. Then, anesthetized domestic swine (weight 25±5 kg, 2 months old) were implanted 25 EPC-capture stents (n=13) and 25 polymer-coated stents (n=13) into left coronary artery. The arteries were harvested at 14 days after the stenting. Histological analysis was performed by hematoxylin-eosin staining and stent strut surface were assessed by electron microscopy. In morphometric analysis, each layer of vascular wall was calculated in the section. Procedural success was achieved in all swine and all stents were patent without thrombosis. Electron microscopy suggest that uniformly endothelialization at the stent strut surface, and hematoxylin-eosin staining showed that neointimal thickining were thin and uniformly.

从外周血单个核细胞中分离人内皮祖细胞（EPS）标志物阳性细胞，采用真实的RT-PCR方法检测其mRNA水平。人单个核细胞中有少量KDR阳性细胞。在培养后第14至21天鉴定内皮集落形成细胞（早期EPS）集落。我们用荧光显微镜对早期EPC进行了免疫分型，并用非定量RT-PCR对早期EPS进行了表征。我们建立了鉴定EPS的实验方法。然后，将25±5 kg体重的2月龄麻醉家猪（n=13）植入左冠状动脉内25个EPC捕获支架（n=13）和25个聚合物涂层支架（n=13）。支架植入后14天取动脉。通过苏木精-伊红染色进行组织学分析，并通过电子显微镜评估支架支柱表面。在形态计量学分析中，计算切片中血管壁的每一层。所有猪均获得手术成功，所有支架均通畅，无血栓形成。电镜显示支架支柱表面均匀内皮化，苏木精-伊红染色显示新生内膜厚度薄且均匀。

项目成果

Activation of cardiac progenitor cells through paracrine effects of mesenchymal stem cells

• DOI: 10.1016/j.bbrc.2008.06.074
• 发表时间: 2008-09-12
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Nakanishi, Chiaki;Yamagishi, Masakazu;Nagaya, Noritoshi
• 通讯作者: Nagaya, Noritoshi