A genetic biochemical search and analysis for factors involved in glycosphingolipid metabolism and traffic

我们使用志贺毒素进行遗传生化筛选，以寻找影响鞘糖脂(GSL)代谢及其细胞内运输的新因素。作为志贺毒素受体Gb3的还原分子，我们分离了TMBIM家族成员grina的COOH末端区域，命名为grina-C。GRINA-C和另一个TMBIM家族成员FAIM2的过表达影响了Gb3合成酶的数量及其在细胞内的定位，提示了糖基转移酶在GSL代谢中的一种新的调节方式。

We performed genetic biochemical screenings with shiga toxin to look for new factors that affect glycosphingolipid (GSL) metabolism and its intracellular traffic. As a molecule to reduce Gb3, the shiga toxin receptor, we isolated the COOH-terminus region of GRINA, a member of TMBIM family, which was named GRINA-C. Overexpression of GRINA-C and FAIM2, another TMBIM family member, affected the amount of Gb3 synthase and its intracellular localization, suggesting a new type of regulation of glycosyltransferases in GSL metabolism.