Structure analysis of cytoplasmic dynein stalk domain

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In order to reveal the recognition mechanism of the microtubule by motor protein dynein, I have searched the crystallization condition of the mouse dynein stalk domain and could obtain single crystals. I prepared the selenomethionine-substituted protein and collected the diffraction data of the crystals using Synchrotron Radiation (2.8 A resolution). Initial phase are calculated by the multiwavelength anomalous dispersion (MAD) method, I could obtain the partial model of the protein (coiled-coil region). The model revealed that the positions of the methionine, which are calculated by using the anomalous dispersion signal of the selenium, are shifted those of the reported crystal structure.