Structure analysis of cytoplasmic dynein stalk domain

细胞质动力蛋白茎结构域的结构分析

• 批准号: 20770118
• 负责人: SHIBA Tomoo
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770118/
• 关键词: モーター蛋白質; X 線結晶構造解析; 多波長異常分散法; セレノメチオニン; ダイニン; 微小官; X線結晶構造解析

In order to reveal the recognition mechanism of the microtubule by motor protein dynein, I have searched the crystallization condition of the mouse dynein stalk domain and could obtain single crystals. I prepared the selenomethionine-substituted protein and collected the diffraction data of the crystals using Synchrotron Radiation (2.8 A resolution). Initial phase are calculated by the multiwavelength anomalous dispersion (MAD) method, I could obtain the partial model of the protein (coiled-coil region). The model revealed that the positions of the methionine, which are calculated by using the anomalous dispersion signal of the selenium, are shifted those of the reported crystal structure.

为了揭示动力蛋白对微管的识别机制，本研究对小鼠动力蛋白柄区的结晶条件进行了探索，获得了动力蛋白柄区的单晶。我制备了硒代蛋氨酸取代的蛋白质，并使用同步辐射（2.8 A分辨率）收集了晶体的衍射数据。用多波长反常色散（MAD）方法计算初始相位，得到蛋白质的部分模型（卷曲螺旋区）。该模型揭示了蛋氨酸的位置，这是通过使用硒的异常色散信号计算，移动那些报道的晶体结构。

项目成果

Crystallization and preliminary crystallographic analysis of cyanide-insensitive alternative oxidase from Trypanosoma brucei brucei

布氏锥虫对氰化物不敏感的替代氧化酶的结晶和初步晶体学分析

• 发表时间: 2010
• 期刊: Acta Crystallographica F66
• 作者: Kido;Y.;et al.
• 通讯作者: et al.

Crystallographic studies of ferredoxin-NAD(P)+ reductase from Chlorobium tepidum

来自温绿藻的铁氧还蛋白-NAD(P) 还原酶的晶体学研究

• 发表时间: 2008
• 作者: Norifumi Muraki;Tomoo Shiba;Daisuke Seo;Takeshi Sakurai and Genji Kurisu
• 通讯作者: Takeshi Sakurai and Genji Kurisu

Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products:: Atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction

• DOI: 10.1021/bi800413r
• 发表时间: 2008-10-14
• 期刊: BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Inaoka, Daniel Ken;Sakamoto, Kimitoshi;Harada, Shigeharu
• 通讯作者: Harada, Shigeharu

Crystallization and preliminary X-ray studies of the chromophorebinding domain of

发色团结合结构域的结晶和初步 X 射线研究

• 发表时间: 2009
• 期刊: Acta Crystallographica Section F F65
• 作者: Rei Narikawa;Norifumi Muraki;T
• 通讯作者: T

Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of Trypanosoma brucei gambiense glycerol kinase

• DOI: 10.1107/s1744309110000369
• 发表时间: 2010-03-01
• 期刊: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
• 影响因子: 0.9
• 作者: Balogun, Emmanuel Oluwadare;Inaoka, Daniel Ken;Kita, Kiyoshi
• 通讯作者: Kita, Kiyoshi