Functional analysis of lysosomal protein trafficking in the fruit fly, Drosophila Melanogaster.

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Intracellular trafficking of integral membrane proteins requires formation of transport carriers to reach their final destination. Clathrin coat is a major coatmer responsible for generation of transport vesicles at the post-Golgi compartments. Clathrin adaptors such as GGA and AP-1 function at the Golgi membrane to recruit clathrin from the cytoplasm to the site of clathrin coated vesicle formation. This study was aimed to dissect the functions of GGA and AP-1 using fly cultured cells as a model system for mammals. We carried out the functional characterization of these clathrin adaptors and found that the molecular functions of the clathrin adaptors are highly conserved from fly to mammals.