Morphological and biochemical characterization of the Ebola virus NP-RNA complexes

埃博拉病毒 NP-RNA 复合物的形态和生化特征

• 批准号: 20780217
• 负责人: NODA Takeshi
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780217/
• 关键词: 人獣共通感染症; エボラウイルス; ヌクレオカプシド; 電子顕微鏡; エポラウイルス; nucleopTotein; 電子顕微鏡解析

When Ebola virus nucleoprotein (NP) is expressed in mammalian cells, it assembles into helical structures. Here, the recombinant NP helix purified from cells expressing NP was characterized biochemically and morphologically. We found that the recombinant NP helix is associated with non-viral RNA which is not protected from RNase digestion and that the morphology of the helix changes depending on the environmental salt concentration. The N-terminal 450 amino acid residues of NP are sufficient for these properties. However, digestion of the NP-associated RNA eliminates the plasticity of the helix, suggesting that this RNA is an essential structural component of the helix, binding to individual NP molecules via the N-terminal 450 amino acids. These findings enhance our knowledge of Ebola virus assembly and understanding of the Ebola virus life cycle.

当埃博拉病毒核蛋白（NP）在哺乳动物细胞中表达时，它组装成螺旋结构。在这里，从表达NP的细胞中纯化的重组NP螺旋进行了生化和形态学表征。我们发现，重组NP螺旋是与非病毒RNA，这是不受保护的RNase消化和螺旋的形态变化取决于环境的盐浓度。NP的N-末端450个氨基酸残基足够用于这些性质。然而，NP相关RNA的消化消除了螺旋的可塑性，这表明该RNA是螺旋的基本结构组分，通过N-末端450个氨基酸与单个NP分子结合。这些发现增强了我们对埃博拉病毒组装的了解和对埃博拉病毒生命周期的理解。

项目成果

Emergence and pandemic potential of swine-origin H1N1 influenza virus.

• DOI: 10.1038/nature08157
• 发表时间: 2009-06-18
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Neumann, Gabriele;Noda, Takeshi;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses.

• DOI: 10.1038/nature08260
• 发表时间: 2009-08-20
• 期刊: Nature
• 影响因子: 64.8

Characterization of the Ebola virus nucleoprotein-RNA complex.

埃博拉病毒核蛋白-RNA复合物的表征。

• DOI: 10.1099/vir.0.019794-0
• 发表时间: 2010-06
• 期刊: JOURNAL OF GENERAL VIROLOGY
• 影响因子: 3.8
• 作者: Noda, Takeshi;Hagiwara, Kyoji;Sagara, Hiroshi;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

Inhibition of Lassa and Marburg Virus Production by Tetherin

• DOI: 10.1128/jvi.01607-08
• 发表时间: 2009-03-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Sakuma, Toshie;Noda, Takeshi;Yasuda, Jiro
• 通讯作者: Yasuda, Jiro

ウイルス粒子形成機構の電子顕微鏡解析

电镜分析病毒颗粒形成机制

• 发表时间: 2008
• 作者: 本泉周一郎;中村精一;南部寿則;橋本俊一;野田岳志
• 通讯作者: 野田岳志