Genome side screening of autophagy to elucidate its regulation

自噬的基因组侧面筛选以阐明其调控

• 批准号: 21570197
• 负责人: NODA Takeshi
• 金额: $ 3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570197/
• 关键词: オートファジー; 酵母; スクリーニング; ゲノムワイドスクリーン; タンパク質分解; Tor

Autophagy is intracellular bulk degradation system, but its regulatory mechanism is largely unknown. In this study, we succeeded in introducing ALP system, which enables quantitation of autophagy activity, into the genome wide yeast mutant collection. We measured autophagic activity under nitrogen starvation in large scale and found that several intriguing factors are directly associated with autophagy regulation. Thus we advanced understanding the mechanism of autophagy regulation.

自噬是细胞内体积降解系统，但其调控机制尚不清楚。在本研究中，我们成功地将能够定量自噬活性的ALP系统引入到全基因组酵母突变体中。我们大规模测量了氮饥饿下的自噬活性，发现了几个有趣的因素与自噬调节直接相关。从而进一步了解自噬调控的机制。

项目成果

Rubicon and PLEKHM1 negatively regulate the endocytic/autophagic pathway via a novel Rab7-binding domain.

• DOI: 10.1091/mbc.e10-06-0495
• 发表时间: 2010-12
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: Tabata K;Matsunaga K;Sakane A;Sasaki T;Noda T;Yoshimori T
• 通讯作者: Yoshimori T

The LC3 recruitment mechanism is separate from Atg9L1-dependent membrane formation in the autophagic response against Salmonella.

• DOI: 10.1091/mbc.e10-11-0893
• 发表时间: 2011-07-01
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: Kageyama S;Omori H;Saitoh T;Sone T;Guan JL;Akira S;Imamoto F;Noda T;Yoshimori T
• 通讯作者: Yoshimori T

How is the Membrane Biogenesis Regulated in Autophagy?

自噬中膜生物发生是如何调节的？

• 发表时间: 2010
• 作者: Noda;T.
• 通讯作者: T.

Modulation of Local PtdIns3P Levels by the PI Phosphatase MTMR3 Regulates Constitutive Autophagy

• DOI: 10.1111/j.1600-0854.2010.01034.x
• 发表时间: 2010-04-01
• 期刊: TRAFFIC
• 影响因子: 4.5
• 作者: Taguchi-Atarashi, Naoko;Hamasaki, Maho;Noda, Takeshi
• 通讯作者: Noda, Takeshi