Genome side screening of autophagy to elucidate its regulation

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Autophagy is intracellular bulk degradation system, but its regulatory mechanism is largely unknown. In this study, we succeeded in introducing ALP system, which enables quantitation of autophagy activity, into the genome wide yeast mutant collection. We measured autophagic activity under nitrogen starvation in large scale and found that several intriguing factors are directly associated with autophagy regulation. Thus we advanced understanding the mechanism of autophagy regulation.