Transcriptional regulation of orexin by candidate genes for narcolepsy

发作性睡病候选基因对食欲素的转录调节

• 批准号: 20790868
• 负责人: TANAKA Susumu
• 金额: $ 2.75万
• 依托单位: Tokyo Metropolitan Organization for Medical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790868/
• 关键词: ナルコレプシー; ORX; IGFBP3; NR6A1; 転写制御; オレキシン; Nur77; 睡眠

In this study, I examined the activities of transcriptional modulations of two narcolepsy candidate genes, IGFBP3 and NR6A1, against ORX transcription. (1)IGFBP3 and NR6A1 expression were found in hypothalamic ORX neurons by immunehistochemical studies. (2)By using reporter assay, IGFBP3 and NR6A1 modulated ORX promoter activity in several cell lines. (3)IGFBP3 overexpression mice showed the reduction of hypothalamic ORX expression and modulated sleep conditions. I also found NR6A1 overexpression modulated ORX expression, however the mice number examined have not reached statistically enough yet.

在这项研究中，我检查了两个发作性睡病候选基因IGFBP3和NR6A1对ORX转录的转录调节活性。（1）免疫组化结果显示IGFBP3和NR6A1在下丘脑ORX神经元表达。(2)By利用报告基因分析，IGFBP3和NR6A1在几种细胞系中调节ORX启动子活性。（3）IGFBP3过表达小鼠下丘脑ORX表达减少，睡眠状态改变。我还发现NR6A1过表达调节ORX的表达，但是检测的小鼠数量还没有达到统计学上足够的水平。

项目成果

ラット視床下部におけるオレキシンmRNAの日内変動パターン

大鼠下丘脑食欲素 mRNA 的昼夜模式

• 发表时间: 2009
• 作者: 田中進;児玉亨;本多芳子;臼井節夫;本多真
• 通讯作者: 本多真

HLA gene expression in white blood cells of narcolepsy.

发作性睡病白细胞中 HLA 基因的表达。

• 发表时间: 2009
• 作者: Honda M;Tanaka S;Honda Y
• 通讯作者: Honda Y

Polymorphism located in TCRA locus confers susceptibility to essential hypersomnia with HLA-DRB1*1501-DQB1*0602 haplotype

• DOI: 10.1038/jhg.2009.118
• 发表时间: 2010-01-01
• 期刊: JOURNAL OF HUMAN GENETICS
• 影响因子: 3.5
• 作者: Miyagawa, Taku;Honda, Makoto;Tokunaga, Katsushi
• 通讯作者: Tokunaga, Katsushi

Isotype analysis of human anti-Borna disease virus antibodies in Japanese psychiatric and general population

• DOI: 10.1016/j.jcv.2008.07.011
• 发表时间: 2008-11-01
• 期刊: JOURNAL OF CLINICAL VIROLOGY
• 影响因子: 8.8
• 作者: Matsunaga, Hidenori;Tanaka, Susumu;Takeda, Masatoshi
• 通讯作者: Takeda, Masatoshi

Polymorphism located between CPT1B and CHKB, and HLA-DRB1*1501-DQB1*0602 haplotype confer susceptibility to CNS hypersomnias (essential hypersomnia).

• DOI: 10.1371/journal.pone.0005394
• 发表时间: 2009
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Miyagawa T;Honda M;Kawashima M;Shimada M;Tanaka S;Honda Y;Tokunaga K
• 通讯作者: Tokunaga K