Identification of the transcription factors for hypothalamic orexin

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In this study, we tried to identify the transcription factors for hypothalamic orexin transcription.By using One-hybrid system, we found some transcription factors binds to II3 sequence in orexin regulatory element 1. We found that one of positive clones, clone 111, expresses in embryonic lateral hypothalamus as well as orexin. Clone 111 moderates the activity of orexin upstream region in reporter assay. Clone 111 co-expresses in orexin neurons of adult hypothalamus. Clone 111 has no strong association with narcolepsy in genome wide association study.Further studies using clone 111 knock-out mouse are needed to show the involvement of clone 111 against orexin transcription.