Identification of the transcription factors for hypothalamic orexin

在本研究中，我们试图鉴定与下丘脑食欲素转录相关的转录因子。通过单杂交系统，我们发现一些转录因子与食欲素调节元件1中的II3序列结合。我们发现其中一个阳性克隆克隆111在胚胎下丘脑的外侧核中表达食欲素。在报告实验中，克隆111调节了增食欲素上游区域的活性。克隆111在成人下丘脑的增食欲素神经元中共表达。在全基因组关联研究中，111号克隆与发作性睡病没有很强的相关性，还需要用111号克隆敲除小鼠进行进一步的研究，以证明111号克隆参与了对增食欲素转录的抑制。

In this study, we tried to identify the transcription factors for hypothalamic orexin transcription.By using One-hybrid system, we found some transcription factors binds to II3 sequence in orexin regulatory element 1. We found that one of positive clones, clone 111, expresses in embryonic lateral hypothalamus as well as orexin. Clone 111 moderates the activity of orexin upstream region in reporter assay. Clone 111 co-expresses in orexin neurons of adult hypothalamus. Clone 111 has no strong association with narcolepsy in genome wide association study.Further studies using clone 111 knock-out mouse are needed to show the involvement of clone 111 against orexin transcription.