Molecular diagnosis and carcinogenetic risk evaluation for lung cancer using methylation-specific DNA microarray

使用甲基化特异性DNA微阵列进行肺癌分子诊断和致癌风险评估

• 批准号: 20790978
• 负责人: ENDO Makoto
• 金额: $ 2.75万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790978/
• 关键词: メチル化; 肺癌; メチル化診断チップ

We devloped a fiber-type DNA microarray on which methylated sequence probes were mounted, and quantitatively analyzed DNA methylation rates (MRs) of 4 cancer-related genes of CpG island using it. In recurrent group rates of methylated RUNX3 was significantly higher than survivor. Analysis of DNA methylation status using DNA microarray could be a promising method for risk assessment in the recurrence of lung cancer.

我们开发了安装了甲基化序列探针的纤维型DNA微阵列，并使用它定量分析了CpG岛的4个癌症相关基因的DNA甲基化率（MR）。在复发组中，RUNX3 甲基化率显着高于幸存者。使用 DNA 微阵列分析 DNA 甲基化状态可能是评估肺癌复发风险的一种有前途的方法。