Fluorescence diagnosis of lung cancer and malignant mesothelioma using orthotopic ijplamtation SCID mice model

原位 ijplamtation SCID 小鼠模型对肺癌和恶性间皮瘤的荧光诊断

• 批准号: 20790991
• 负责人: TAKIZAWA Hiromitsu
• 金额: $ 2.75万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790991/
• 关键词: 蛍光診断; 5-アミノレブリン酸; タラポルフィン; SCIDマウス; 肺癌; 悪性胸膜中皮腫; リンパ節転移; 光感受性物質

Human lung cancer cell line A549, Ma44-3 and human malignant mesothelioma cell line MST0211H were injected into left lung or left pleural cavity of severe combined immunodeficiency disease mice. The mice were administered 5-aminolevulinic acid or mono-L-aspartyl chlorine e6 1 to 3 weeks after the implantation. The mice were sacrificed several hours after the administration of these drugs. Their chest walls were opened and illuminated with blue light (405nm). Clear red fluorescence was observed in lung tumors, metastatic lymph nodes, and pleural tumors, even though the tumors were invisible macroscopically under white light. This result suggests possibility of clinical application of fluorescence diagnosis with intrapleural malignant tumors.

将人肺癌细胞系A549、Ma44-3和人恶性间皮瘤细胞系MST0211H注射到严重联合免疫缺陷病小鼠的左肺或左胸膜腔中。植入后1至3周给小鼠施用5-氨基乙酰丙酸或单-L-天冬氨酰氯e6。施用这些药物几小时后处死小鼠。他们的胸壁被打开并用蓝光（405nm）照射。在肺肿瘤、转移性淋巴结和胸膜肿瘤中观察到清晰的红色荧光，尽管这些肿瘤在白光下肉眼看不见。这一结果提示了荧光诊断胸膜内恶性肿瘤临床应用的可能性。

项目成果

Fluorescence diagnosis of lymph node metastasis of lung cancer in mouse model.

小鼠肺癌淋巴结转移模型的荧光诊断。

• 发表时间: 2009
• 期刊: Oncology Reports 22
• 作者: Hiromitsu Takizawa;Kazuya Kondo;Hiroaki Toba;Koichiro Kenzaki;Shoji Sakiyama;Akira Tangoku
• 通讯作者: Akira Tangoku

Fluorescence diagnosis of lymph node metastasis of lung cancer in mouse model

小鼠肺癌淋巴结转移模型的荧光诊断

• 发表时间: 2009
• 期刊: Oncology Reports 22
• 作者: Takizawa;et al
• 通讯作者: et al