Fluorescence diagnosis of lung cancer and malignant mesothelioma using orthotopic ijplamtation SCID mice model

点击翻译按钮获取中文摘要

Human lung cancer cell line A549, Ma44-3 and human malignant mesothelioma cell line MST0211H were injected into left lung or left pleural cavity of severe combined immunodeficiency disease mice. The mice were administered 5-aminolevulinic acid or mono-L-aspartyl chlorine e6 1 to 3 weeks after the implantation. The mice were sacrificed several hours after the administration of these drugs. Their chest walls were opened and illuminated with blue light (405nm). Clear red fluorescence was observed in lung tumors, metastatic lymph nodes, and pleural tumors, even though the tumors were invisible macroscopically under white light. This result suggests possibility of clinical application of fluorescence diagnosis with intrapleural malignant tumors.