Establishment of cell therapy for transplanting neural crest-derived stem cells into spinal cord injury model of rodent and mammalian

啮齿动物和哺乳动物脊髓损伤模型移植神经嵴干细胞的细胞疗法的建立

• 批准号: 20791054
• 负责人: NAGOSHI Narihiro
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791054/
• 关键词: 再生医療; 脊髄損傷; 細胞移植; 神経堤幹細胞; 霊長類; 神経堤; 後根神経節; 皮膚; 骨髄; 胚葉転換

Neural crest-derived stem cells (NCSCs) are isolated from adult skin and bone marrow, and they have the ability to differentiate into neural cells. Therefore, NCSCs are expected as cell sources for transplantation into spinal cord injury. In our study, we transplanted NCSCs into spinal cord injury model of mice. We observed the transplanted NCSCs surviving in the injured spinal cord, differentiating into neural cells. Moreover, the transplanted NCSCs had no tumorigenesis, suggesting that transplantation of NCSCs are safe. We also isolated and characterized NCSCs from adult marmoset or human skin.

神经嵴源性干细胞（Neural crest-derived stem cells, NCSCs）是从成人皮肤和骨髓中分离出来的，它们具有向神经细胞分化的能力。因此，NCSCs有望成为脊髓损伤移植的细胞来源。在本研究中，我们将NCSCs移植到小鼠脊髓损伤模型中。我们观察到移植的NCSCs在损伤脊髓中存活并分化为神经细胞。此外，移植的NCSCs没有发生肿瘤，表明NCSCs移植是安全的。我们还从成年狨猴或人类皮肤中分离和鉴定了NCSCs。

项目成果

神経堤幹細胞の発生と多能性-中枢神経の再生を目指して-

神经嵴干细胞的发育和多能性 - 以中枢神经系统再生为目标 -

• 发表时间: 2009
• 作者: 名越慈人;他;名越慈人
• 通讯作者: 名越慈人

Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow

小鼠骨髓中神经嵴干细胞的个体发育和多能性

• 发表时间: 2008
• 作者: Nagoshi N;et.al.
• 通讯作者: et.al.

Characterization of neural crest stem cells as a source of transplantation for spinal cord injury

神经嵴干细胞作为脊髓损伤移植来源的表征

• 发表时间: 2008
• 作者: Nagoshi N;et.al.
• 通讯作者: et.al.

マウス損傷脊髄に対する神経堤幹細胞移植の検討

损伤小鼠脊髓神经嵴干细胞移植检查

• 发表时间: 2008
• 作者: 名越慈人、芝田晋介;他
• 通讯作者: 他

Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow.

小鼠骨髓中多能间充质干细胞的前瞻性鉴定、分离和系统移植。

• DOI: 10.1084/jem.20091046
• 发表时间: 2009-10-26
• 期刊: The Journal of experimental medicine
• 作者: Morikawa S;Mabuchi Y;Kubota Y;Nagai Y;Niibe K;Hiratsu E;Suzuki S;Miyauchi-Hara C;Nagoshi N;Sunabori T;Shimmura S;Miyawaki A;Nakagawa T;Suda T;Okano H;Matsuzaki Y
• 通讯作者: Matsuzaki Y