The Role and Inhibitory Effect of Myostatin for Muscle Regeneration in Human Urethral Sphincter

以慢肌纤维为主的尿道横纹括约肌在持续收缩的尿控机制中起重要作用。因此，尿道横纹括约肌功能障碍导致顽固性尿失禁。本研究通过对骨骼肌肌发生的负性调节因子肌肉抑制素(myostatin，MST)对人尿道横纹括约肌(Hur)卫星细胞(横纹肌干细胞)的生长分化机制进行了研究。MST通过磷酸化Smad-2和细胞周期停滞抑制Hur卫星细胞的增殖。相反，FS抑制了这些效应。另一方面，目前的结果不能表明HUR卫星细胞本身具有MST的自分泌抑制生长的作用。

Urethral rhabdoshincter that mainly consist of slow muscle fibers, have an important role of urinary continence mechanism with continuous contraction. Thus, dysfunction of urethral rhabdoshincter caused intractable urinary incontinence.I this research, we studied the mechanism of growth and differentiation in human urethral rhabdoshincter (HUR) satellite cells (striated muscle stem cells) by myostatin (MST), which was a negative regulator of myogenesis of skeletal muscle. MST inhibited the proliferation of HUR satellite cells through phosphorylated smad-2 and cell cycle arrest. On the contrary, FS suppressed these effects. On the other hand, current results could not indicate that HUR satellite cells had an autocrine action of MST for the growth inhibition by itself.