Proteomics to identify the targets of antiphospholipid antibodies in women with recurrent pregnancy loss

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Blood plasma samples were collected from women with recurrent pregnancy loss. Further, a clinical database of these patients was constructed.Solid-phase plates comprising phospholipids embedded within cell membranes were constructed to extract phospholipids that bind to plasma proteins. The blood plasma samples were reacted with the plates to isolate the target plasma proteins. These proteins were visualized by 2-dimensional electrophoresis (2-DE). The protein spots on the 2-DE gels were identified by mass spectrometry.