Induction of corneal endothelial cells from human embryonic stem cells based on the analysis of molecular markers during corneal endothelial development

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We generated the knock-in mice expressing a green fluorescent protein (GFP) in mesencephalic neural crest cells and their derivatives. The analysis of molecular markers during corneal endothelial development was performed using the knock-in mice. The in vitro induction of corneal endothelium from mouse embryonic stem cells was developed based on the molecular markers to mimic the mouse corneal endothelial development. The protocol to produce corneal endothelium from mouse embryonic stem cells was applied to in vitro induction of corneal endothelium from human embryonic stem cell. This research shed light on the production of the cellular source for the corneal endothelial transplantation.