Basic research of the development of new pulp capping agent

磷酸蛋白是牙本质矿化过程中的重要蛋白质。从猪牙中提取，并与1型胶原复合作为试验材料。将该试验材料置于8周龄Wistar大鼠上颌第一磨牙的暴露牙髓上。术后1-3周处死大鼠，用苏木精-伊红染色牙齿。在评价修复性牙本质形成和牙髓炎症时，将染色切片与普通材料氢氧化钙进行比较。磷酸化蛋白-胶原复合物可诱导良好的修复性牙本质，牙髓炎症轻微。因此，该材料被认为具有作为氢氧化钙的替代材料的潜力。

Phosphophoryn is an important protein in dentin mineralization. It was extracted from pig teeth, and was combined with type 1 collagen as a trial material. This trial material was placed onto the exposed pulp in maxillary first molar of 8 week-old Wistar rats. Rats were sacrificed 1-3 weeks after operation, and teeth were stained with hematoxylin-eosin. In evaluation of reparative dentin formation and pulp inflammation, the stained sections were compared with calcium hydroxide, a common material. The phosphophoryn-collagen complex induced good reparative dentin, and dental pulp inflammation was mild. Therefore, this material was suggested to have a potential as an alternative material of calcium hydroxide.