Delivery of siRNA to central nervous system viablood-brain barrirer transport : Therapy for prion disease

通过血脑屏障转运将 siRNA 递送至中枢神经系统：朊病毒病的治疗

• 批准号: 20800066
• 负责人: WATANABE Takuya
• 金额: $ 2.11万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20800066/
• 关键词: 薬学; 感染症

This study aimed to develop new therapy for prion diease without side effects, using a complex of siRNA and RVG-9R. The siRNA decreased expression of prion mRNA and prion protein of scrapie form (PrP^<Sc>). And siRNA/RVG-9R complex bound to cell. However, treatment of siRNA/RVG-9R complex did not affect PrP^<Sc> level in prion-infected cell culture. Sequence of RVG-9R needs to optimize for knock-down of prion.

本研究的目的是开发一种新的治疗朊病毒病的无副作用，使用siRNA和RVG-9 R的复合物。siRNA可降低羊瘙痒症型朊病毒mRNA和朊病毒蛋白（PrP^）的表达<Sc>。并且siRNA/RVG-9 R复合物与细胞结合。然而，siRNA/RVG-9 R复合物的处理不影响朊<Sc>病毒感染的细胞培养物中的PrP β 2水平。RVG-9 R的序列需要优化以敲除朊病毒。

项目成果

Lipopolysaccharide-Activated Microglia Induce Dysfunction of the Blood-Brain Barrier in Rat Microvascular Endothelial Cells Co-Cultured with Microglia

• DOI: 10.1007/s10571-009-9446-7
• 发表时间: 2010-03-01
• 期刊: CELLULAR AND MOLECULAR NEUROBIOLOGY
• 影响因子: 4
• 作者: Sumi, Noriko;Nishioku, Tsuyoshi;Kataoka, Yasufumi
• 通讯作者: Kataoka, Yasufumi