The role of citric acid cycle and related metabolites in IDH1-mutated gliomas

柠檬酸循环及相关代谢物在 IDH1 突变胶质瘤中的作用

• 批准号: 24791490
• 负责人: WATANABE Takuya
• 金额: $ 2.75万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791490/
• 关键词: 神経膠腫; IDH1; クエン酸サイクル; MRS; 免疫組織化学; MGMT; テモゾロミド

We analyzed the intratumoral metabolism of 19 glioma samples using the LC model that the third party software which can calculate quantitative MR spectrum value of each metabolites. We especially focused on citric acid related metabolites including N-acetyl-aspartate (NAA) and N-acetyl-aspartyl-glutamate (NAAG). Major metabolites in glial cells such as glucose, glutamate acid, GPC did not indicate significant change between IDH-mutated and wild-type glioma. NAA also did not indicate significant differences, whereas NAAG indicated 0.237 (35% reduction) in IDH-mutated glioma comparison to 0.362 in wild type glioma, suggesting that metabolic environment in glioma cells were significantly changed between IDH-mutated and wild-type glioma. Pre-operative MR analysis of glioma using LC model has the possibility of differential diagnosis of IDH-mutated glioma.

我们利用第三方软件LC模型对19例胶质瘤标本进行了瘤内代谢分析，该模型可以计算每种代谢物的定量MR谱值。我们特别关注柠檬酸相关的代谢物，包括N-乙酰天冬氨酸（NAA）和N-乙酰乙酰谷氨酸（NAAG）。胶质细胞内的主要代谢产物如葡萄糖、谷氨酸、GPC在IDH突变型和野生型胶质瘤之间没有显著变化。NAA也没有显示显著差异，而NAAG显示IDH突变的胶质瘤中的0.237（35%降低）与野生型胶质瘤中的0.362相比，表明胶质瘤细胞中的代谢环境在IDH突变的胶质瘤和野生型胶质瘤之间显著改变。术前应用LC模型对胶质瘤进行MR分析有可能对IDH突变胶质瘤进行鉴别诊断。

项目成果

GSK3β阻害作用を有する既存薬剤を用いた再発膠芽腫の免疫組織学的検討．

使用抑制 GSK3β 的现有药物对复发性胶质母细胞瘤进行免疫组织学研究。

• 发表时间: 2013
• 作者: Takeo Shimasaki;Toshinari Minamoto;et al.;宮下勝吉，源 利成，ほか．
• 通讯作者: 宮下勝吉，源 利成，ほか．

Visualization of angiographical arteriovenous shunting in perisylvian glioblastomas

• DOI: 10.1007/s00701-013-1650-z
• 发表时间: 2013-04-01
• 期刊: ACTA NEUROCHIRURGICA
• 影响因子: 2.4
• 作者: Yoshikawa, A.;Nakada, M.;Hayashi, Y.
• 通讯作者: Hayashi, Y.

Progressive adult primary glioblastoma in the medulla oblongata with an unmethylated MGMT promoter and without an IDH mutation

• DOI: 10.1007/s10014-012-0118-9
• 发表时间: 2013-07-01
• 期刊: BRAIN TUMOR PATHOLOGY
• 影响因子: 3.3
• 作者: Yoshikawa, Akifumi;Nakada, Mitsutoshi;Hamada, Jun-ichiro
• 通讯作者: Hamada, Jun-ichiro

Prognostic paradox: brain damage around the glioblastoma resection cavity

• DOI: 10.1007/s11060-014-1418-1
• 发表时间: 2014-05-01
• 期刊: JOURNAL OF NEURO-ONCOLOGY
• 影响因子: 3.9
• 作者: Furuta, Takuya;Nakada, Mitsutoshi;Hamada, Jun-ichiro
• 通讯作者: Hamada, Jun-ichiro

Advances in experimental medicine and biology, chapter 8, Receptor Tyrosine Kiases: Principles and Functions in Glioma Invasion

实验医学和生物学进展，第 8 章，受体酪氨酸激酶：胶质瘤侵袭的原理和功能

• 发表时间: 2013
• 作者: Mitsutoshi Nakada;Daisuke Kita;Takuya Watanabe;Yutaka Hayashi;Jun-ichiro Hamada
• 通讯作者: Jun-ichiro Hamada