Molecular biological analysis of the Babesia parasite transmission blocking mechanism conducted by tick aminopeptidase.

通过蜱氨肽酶进行巴贝虫寄生虫传播阻断机制的分子生物学分析。

• 批准号: 20880040
• 负责人: HATTA Takeshi
• 金额: $ 2.01万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20880040/
• 关键词: マダニ; アミノペプチダーゼ; バベシア原虫; マダニ媒介感染症

Tick is well known biological vector of zoonotic infectious diseases such as Lyme disease but the tick molecular function regarding pathogen transmission is poorly understood. A terminal enzyme of host blood digestion in tick midgut was an aminopeptidase, HlLAP which was shown to be involved in the regulatory effect on babesial migration in tick by inhibiting its invasion. Based on the cellular biological study, we observed that HlLAP gene silencing by RNA interference induced the immature generation of peritrophic membrane (PM) in midgut. PM is the mechanical barrier of midgut epithelium against bacterial or xenobiotic substances in host blood meal. Therefore, it was assumed that HlLAP which was responsible for the generation of PM disruption of which inhibited indirectly an invasion of Babesia parasite into the midgut epithelium.

蜱是莱姆病等人畜共患传染病的重要传播媒介，但蜱在病原体传播中的分子功能尚不清楚。蜱类中肠消化宿主血液的末端酶是一种氨肽酶，Hlt3参与调节蜱类巴贝斯虫的迁移，抑制其入侵。在细胞生物学研究的基础上，我们观察到RNA干扰沉默HlxB基因可诱导中肠围食膜（PM）的不成熟产生。PM是中肠上皮细胞对宿主血粉中细菌或外源性物质的机械屏障。因此，假设负责PM产生的Hllcadium破坏间接抑制了巴贝虫寄生虫侵入中肠上皮。

项目成果

バベシア原虫のマダニ介卵伝播におけるマダニアミノペプチダーゼの役割

蜱氨肽酶在蜱介导的卵介导的巴贝虫原生动物传播中的作用

• 发表时间: 2009
• 作者: Okamoto N;Tanaka A;Jung K;Karasawa K;Orito K;Matsuda A;Amagai Y;Oida K;Ohmori K;Matsuda H;八田岳士,辻尚利,三好猛晴,山地佳代子,Anisuzzaman,藤崎幸藏
• 通讯作者: 八田岳士,辻尚利,三好猛晴,山地佳代子,Anisuzzaman,藤崎幸藏

ロイシンアミノペプチダーゼ遺伝子抑制マダニにおけるBabesia gibsoni介卵伝搬効率の上昇

亮氨酸氨肽酶基因抑制可提高蜱中吉氏巴贝虫卵的传播效率

• 发表时间: 2008
• 作者: Y.Sakurai;H.Hatakeyama;et al.;八田岳士
• 通讯作者: 八田岳士

マダニアレイ解析:マダニにおけるバベシア原虫の介卵伝播に関わる生物活性分子の探索

蜱阵列分析：寻找参与蜱虫卵介导的巴贝虫原虫传播的生物活性分子

• 发表时间: 2008
• 作者: Kondo;Y.;Sasaki;T.;Sato;Y.;Amano;A.;Iwama;M.;Handa;S.;Shimada;N.;Fukuda;M.;Akita;M.;Lee;J.;Jeong;KS.;Maruyama;N.;Ishigami;A.;八田岳士
• 通讯作者: 八田岳士

Leucine aminopeptidase, HlLAP, from the ixodid tick Haemaphysalis longicornis, plays vital roles in the development of oocytes

• DOI: 10.1016/j.parint.2010.03.001
• 发表时间: 2010-06-01
• 期刊: PARASITOLOGY INTERNATIONAL
• 影响因子: 1.9
• 作者: Hata, Takeshi;Tsuji, Naotoshi;Fujisaki, Kozo
• 通讯作者: Fujisaki, Kozo

マダニのバベシア原虫感染発育胚にけるアミノぺプチダーゼ遺伝子の発現応答

蜱巴贝斯虫感染发育胚胎中氨肽酶基因的表达反应

• 发表时间: 2009
• 作者: Arai;Y.K.;et al;八田岳士
• 通讯作者: 八田岳士