Study for developing information of cis-elements related to regulation of gene expression in rice

水稻基因表达调控相关顺式元件信息开发研究

• 批准号: 20880041
• 负责人: SATO Yutaka
• 金额: $ 2.11万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20880041/
• 关键词: 遺伝子発現; シス配列; マイクロアレイ; 転写因子; イネ

In order to construct an analysis tool for identifying cis-elements related to regulation of gene expression, we extracted the sequences of putative promoter regions of all annotated genes in rice, which correspond to the 1kb and 2kb from the start point of transcription for each gene, and performed a wide range of co-expression analyses. Furthermore, we designed probes for protein binding microarray in order to extract the sequence, with which a transcription factor directly interact.

为了构建一个鉴定基因表达调控相关顺式元件的分析工具，我们提取了水稻中所有注释基因的推定启动子区域序列，分别对应于每个基因转录起始点的1kb和2kb，并进行了广泛的共表达分析。此外，我们设计了蛋白质结合微阵列探针，以提取与转录因子直接相互作用的序列。