Study for developing information of cis-elements related to regulation of gene expression in rice

水稻基因表达调控相关顺式元件信息开发研究

• 批准号: 20880041
• 负责人: SATO Yutaka
• 金额: $ 2.11万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20880041/
• 关键词: 遺伝子発現; シス配列; マイクロアレイ; 転写因子; イネ

In order to construct an analysis tool for identifying cis-elements related to regulation of gene expression, we extracted the sequences of putative promoter regions of all annotated genes in rice, which correspond to the 1kb and 2kb from the start point of transcription for each gene, and performed a wide range of co-expression analyses. Furthermore, we designed probes for protein binding microarray in order to extract the sequence, with which a transcription factor directly interact.

为了构建一种分析工具，用于识别与基因表达调节有关的顺式元素，我们从每个基因的转录开始点提取了大米中所有注释基因的假定启动子区域的序列，它们对应于1KB和2KB，并进行了广泛的共表达分析。此外，我们为蛋白质结合微阵列设计了探针，以提取转录因子直接相互作用的序列。