Contemporary reconsideration of bacterial infectious using pathological section : Verification of blind spot of bacteria test

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We examined the situation of clinically undetected or overlooked bacterial infection caused by general bacteria from the viewpoint of pathological diagnosis. We created a bacterial colony sample panel and established histochemical detection conditions in the pathology sample. A specific positive signal could be detected in all examples except for E. coli. The result of clinical bacterial isolate and immunostaining in the pyuria cell block specimen was consistent in 10/12 examples. The in situ hybridization method was negative in all examples ; therefore it is necessary to consider changing to a probe with more efficient detection.