Analysis of phosphorylation cascade of molecules expressed in podocyte as the pathophysiological basis of kidney diseases

点击翻译按钮获取中文摘要

In this study, we demonstrated and reported the following findings.1) Phosphorylation of adhesion molecules of podocyte, such as neph1 is related to the development of proteinuria.2) Nephrin binds to PLC-γ1 through its phosphorylation and it evokes the increase of intracellular calcium concentration([Ca2^+] i).3) In adiition, we demonstrated that nephrin regulated the activity of TRPC6 channel on podocyte cytoplasmic membrane, which also regulate([Ca2^<+]> i).4) Finally, we analyzed inherited renal disease, i. e. Epstein syndrome, and revealed that its pathogenesis is FSGS, and myosin right chain IIA was decreased in this disease.These findings are published in, JBC 2008, JBC 2009, KidneyInter 2010, and Mol Cell Biol 2011.